Thomassen M J, Barna B P, Farmer M O, Doershuk C F, Stern R C
Department of Pulmonary Disease, Cleveland Clinic Foundation, Ohio 44195-5038.
Pediatr Pulmonol. 1990;8(3):178-81. doi: 10.1002/ppul.1950080309.
Peripheral blood monocytes were obtained from 19 patients with cystic fibrosis (CF) and age-matched paired normal individuals. The oxidative metabolic response of these cells was measured by superoxide anion production before and after stimulation with Salmonella typhimurium or Pseudomonas aeruginosa lipopolysaccharide (LPS). CF monocytes showed slightly greater spontaneous superoxide anion production (14.1 +/- 2.1 SEM nanomoles superoxide anion/10(6) monocytes/180 min; n = 12) than normal monocytes (9.5 +/- 1.4; n = 12), P = 0.009. No differences between CF and normals were found in LPS-stimulated superoxide anion production (CF = 33.5 +/- 4.6, n = 13; normal = 33.8 +/- 4.2, n = 13). Furthermore, CF monocytes responded to both P. aeruginosa and S. typhimurium LPS stimulation as well as to recombinant interferon-gamma. Superoxide anion production of CF monocytes was comparable in autologous serum and in normal serum, and responses of patients colonized with P. aeruginosa and P. cepacia did not differ. We conclude that CF monocytes have a slightly increased metabolic level and, despite chronic infection, are capable of a further response to exogenous microbial stimuli.
从19例囊性纤维化（CF）患者以及年龄匹配的正常对照个体中获取外周血单核细胞。在用鼠伤寒沙门氏菌或铜绿假单胞菌脂多糖（LPS）刺激前后，通过超氧阴离子生成来测量这些细胞的氧化代谢反应。CF单核细胞的自发超氧阴离子生成量（14.1±2.1 SEM纳摩尔超氧阴离子/10⁶单核细胞/180分钟；n = 12）略高于正常单核细胞（9.5±1.4；n = 12），P = 0.009。在LPS刺激后的超氧阴离子生成方面，未发现CF患者与正常对照之间存在差异（CF = 33.5±4.6，n = 13；正常 = 33.8±4.2，n = 13）。此外，CF单核细胞对铜绿假单胞菌和鼠伤寒沙门氏菌LPS刺激以及重组干扰素-γ均有反应。CF单核细胞在自体血清和正常血清中的超氧阴离子生成相当，并且感染铜绿假单胞菌和洋葱伯克霍尔德菌的患者的反应没有差异。我们得出结论，CF单核细胞的代谢水平略有升高，并且尽管存在慢性感染，但仍能够对外源微生物刺激产生进一步反应。
