Microbiology Section, Department of Biochemistry and Biological Science, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, San Luis, Argentina.
Mycoses. 2011 Nov;54(6):e718-25. doi: 10.1111/j.1439-0507.2010.02006.x. Epub 2011 May 25.
Larrea divaricata Cav. (jarilla) is a plant with well-documented applications in folk medicine in Argentina. In this study, we aimed to evaluate functional parameters of peritoneal macrophages isolated from mice injected with three fractions (F1, F2 and F3) of L. divaricata. The response of macrophages against Candida albicans was evaluated. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test, apoptosis was evaluated using Giemsa, acridine orange/ethidium bromide and ladder assay, oxidative burst was assayed using nitroblue tetrazolium test and nitrite production using Griess assay. Cell stimulation and their ability to kill C. albicans in vitro were measured. The number and cell viability were similar to controls. However, we found that F1 induces pre-activation of macrophages, and this pre-activation is enhanced by C. albicans. The effects exerted by F1 make it more important than F2 and F3 for the treatment of disseminated candidiasis in patients with immunodeficiency diseases such as AIDS and chronic granulomatous disease, among others.
拉雷亚分裂卡特瓦（ Jarilla ）是一种在阿根廷民间医学中有明确应用的植物。在这项研究中，我们旨在评估从注射拉雷亚分裂卡特瓦的三种馏分（ F1 、 F2 和 F3 ）的小鼠中分离的腹腔巨噬细胞的功能参数。评估了巨噬细胞对白色念珠菌的反应。使用 3-（ 4 ， 5-二甲基噻唑-2-基）-2 ， 5-二苯基四氮唑溴盐（ MTT ）试验评估细胞活力，使用吉姆萨染色，吖啶橙/溴化乙锭和梯状试验评估细胞凋亡，使用硝基四氮唑蓝试验评估氧化爆发，使用格里斯测定法测定亚硝酸盐的产生。测量细胞刺激和体外杀死白色念珠菌的能力。细胞数量和活力与对照相似。但是，我们发现 F1 诱导巨噬细胞的前激活，并且这种前激活被白色念珠菌增强。与 F2 和 F3 相比， F1 的作用对于治疗 AIDS 和慢性肉芽肿病等免疫缺陷疾病患者的播散性念珠菌病更为重要。
