Simmons N L
Department of Physiological Sciences, School of Biomedical Science, University of Newcastle upon Tyne.
Exp Physiol. 1990 May;75(3):309-19. doi: 10.1113/expphysiol.1990.sp003406.
A permanently transformed cell line derived from human embryo renal cortical cells (HEK293) has been investigated for the retention of renal-specific properties. The cell line is epithelioid in growth on plastic, and transmission electron microscopy demonstrates the formation of apical zonae occludentes. There is no prominent brush-border. The response of HEK293 cell adenylate cyclase is noteworthy for the response to vasoactive intestinal peptide (half-maximal activation at 0.9 nM). The HEK adenylate cyclase response to VIP is specific, with related peptides such as glucagon and secretin being ineffective. The response to VIP is competitively antagonized by the VIP receptor antagonist (4Cl-D-Phe6,Leu17)-VIP.
对源自人胚胎肾皮质细胞（HEK293）的永久转化细胞系进行了肾特异性特性保留的研究。该细胞系在塑料上生长时呈上皮样，透射电子显微镜显示顶端紧密连接的形成。没有明显的刷状缘。HEK293细胞腺苷酸环化酶对血管活性肠肽的反应值得注意（在0.9 nM时达到半数最大激活）。HEK腺苷酸环化酶对VIP的反应具有特异性，胰高血糖素和促胰液素等相关肽无效。VIP受体拮抗剂（4Cl-D-Phe6，Leu17）-VIP竞争性拮抗对VIP的反应。
