血管活性肠肽对人肾腺苷酸环化酶的体外刺激作用。
Vasoactive intestinal peptide stimulation of human renal adenylate cyclase in vitro.
作者信息
Charlton B G, Neal D E, Simmons N L
机构信息
Department of Physiological Sciences, the Medical School, University of Newcastle upon Tyne, NE2 4HH.
出版信息
J Physiol. 1990 Apr;423:475-84. doi: 10.1113/jphysiol.1990.sp018034.
Abstract
- A direct action of vasoactive intestinal peptide (VIP) upon human kidney was sought by measurement of renal adenylate cyclase in tissue homogenates and plasma membranes isolated from tissue samples excised for therapeutic reasons. 2. VIP (1 microM) produced a mean stimulation of adenylate cyclase activity of 3.5-fold compared to basal values in cortical plasma membranes; comparative stimulations of 2.8-fold and 27.3-fold were obtained with 1 microM-glucagon and 1 microM-h(1-34) parathyroid hormone respectively. 3. Half-maximal stimulation of human renal cortical plasma membrane adenylate cyclase was observed with a mean value of 35 nM-VIP. 4. The stimulation of renal adenylate cyclase by VIP appeared to be specific because stimulation by glucagon was additive to that obtained with VIP, and the VIP receptor antagonist (4 Cl-D-Phe6, Leu17)-VIP inhibited the VIP-dependent stimulation of adenylate cyclase activity.
摘要
- 通过测量从因治疗原因切除的组织样本中分离出的组织匀浆和质膜中的肾腺苷酸环化酶，来探寻血管活性肠肽（VIP）对人肾脏的直接作用。2. 与皮质质膜中的基础值相比，1微摩尔VIP使腺苷酸环化酶活性平均刺激了3.5倍；1微摩尔胰高血糖素和1微摩尔人甲状旁腺激素（1 - 34）分别产生了2.8倍和27.3倍的相对刺激。3. 观察到人肾皮质质膜腺苷酸环化酶的半数最大刺激时，VIP的平均值为35纳摩尔。4. VIP对肾腺苷酸环化酶的刺激似乎具有特异性，因为胰高血糖素的刺激与VIP的刺激具有加和性，并且VIP受体拮抗剂（4 - 氯 - D - 苯丙氨酸6，亮氨酸17）-VIP抑制了依赖VIP的腺苷酸环化酶活性刺激。
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