Abusrewil Sumaya, Scott J Alun, Alqahtani Saeed S, Butcher Mark C, Tiba Mohammed, Kumar Charchit, Mulvihill Daniel M, Ramage Gordon, McLean William
Glasgow Endodontology Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, Glasgow G12 8QF, UK.
Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, 378 Sauchiehall Street, Glasgow G2 3JZ, UK.
Dent J (Basel). 2024 Apr 10;12(4):100. doi: 10.3390/dj12040100.
A tricalcium silicate-based cement, Biodentine™, has displayed antibiofilm activity when mixed with chitosan powder. This study aimed to assess the effect of chitosan incorporation on the physico-mechanical and biological properties of Biodentine™.
In this study, medium molecular weight chitosan powder was incorporated into Biodentine™ in varying proportions (2.5 wt%, 5 wt%, 10 wt%, and 20 wt%). The setting time was determined using a Vicat apparatus, solubility was assessed by calculating weight variation after water immersion, radiopacity was evaluated and expressed in millimeters of aluminum, the compressive strength was evaluated using an Instron testing machine, and the microhardness was measured with a Vickers microhardness tester. In addition, surface topography of specimens was analyzed using scanning electron microscopy, and the effect of chitosan on the viability of human embryonic kidney (HEK 293) cells was measured by a colorimetric MTT assay.
Incorporation of 2.5 wt% and 5 wt% chitosan powder delivered an advantage by speeding up the setting time of Biodentine material. However, the incorporation of chitosan compromised all other material properties and the crystalline structure in a dose-dependent manner. The chitosan-modified material also showed significant decreases in the proliferation of the HEK 293 cells, signifying decreased biocompatibility.
Chitosan incorporation into calcium silicate materials adversely affects the physical and biological properties of the material. Despite the increased antimicrobial activity of the modified material, the diminution in these properties is likely to reduce its clinical value.
一种基于硅酸三钙的骨水泥，即BioDentine™，与壳聚糖粉末混合时已显示出抗生物膜活性。本研究旨在评估壳聚糖掺入对BioDentine™物理力学和生物学性能的影响。
在本研究中，将中分子量壳聚糖粉末以不同比例（2.5 wt%、5 wt%、10 wt%和20 wt%）掺入BioDentine™中。使用维卡仪测定凝结时间，通过计算水浸后重量变化评估溶解度，评估射线不透性并以铝的毫米数表示，使用英斯特朗试验机评估抗压强度，并用维氏显微硬度计测量显微硬度。此外，使用扫描电子显微镜分析标本的表面形貌，并通过比色MTT法测量壳聚糖对人胚肾（HEK 293）细胞活力的影响。
掺入2.5 wt%和5 wt%的壳聚糖粉末通过加快BioDentine材料的凝结时间带来了优势。然而，壳聚糖的掺入以剂量依赖的方式损害了所有其他材料性能和晶体结构。壳聚糖改性材料还显示HEK 293细胞的增殖显著降低，这表明生物相容性降低。
将壳聚糖掺入硅酸钙材料会对材料的物理和生物学性能产生不利影响。尽管改性材料的抗菌活性增加，但这些性能的降低可能会降低其临床价值。
