小鼠造血干细胞和祖细胞的黏附、迁移及归巢
Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors.
作者信息
Cancelas Jose A
机构信息
Division of Experimental Hematology and Cancer Biology, Children's Hospital Medical Center, University of Cincinnati, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2011;750:187-96. doi: 10.1007/978-1-61779-145-1_13.
Abstract
Recent advances in cell biology have demonstrated the role of multiple signaling proteins in the transduction of external signals to cytoplasmic and nuclear effectors, controlling the movement and/or retention of hematopoietic stem cells and progenitors (HSC/P) within the bone marrow, with important clinical implications. Multiple assays have become routine in the analysis of adhesion to the microenvironment, migration toward chemoattractant gradients, and homing of HSC/P in the bone marrow in vivo. In this chapter, we analyze some of the most frequently used assays in our laboratory to explore the ability of HSC to migrate, adhere, and home in in vitro and in vivo assays.
摘要
细胞生物学的最新进展已证明多种信号蛋白在将外部信号转导至细胞质和核效应器中的作用，这些效应器控制着造血干细胞和祖细胞（HSC/P）在骨髓中的移动和/或滞留，具有重要的临床意义。在分析HSC/P与微环境的粘附、向趋化因子梯度的迁移以及在体内骨髓中的归巢时，多种检测方法已成为常规操作。在本章中，我们分析了一些在我们实验室中最常用的检测方法，以探究HSC在体外和体内检测中迁移、粘附和归巢的能力。
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