通过 BOX-PCR 分型和 16S rRNA 基因测序比较环境肠球菌分离株的基因型和系统发育关系。
Comparison of genotypic and phylogenetic relationships of environmental Enterococcus isolates by BOX-PCR typing and 16S rRNA gene sequencing.
机构信息
Department of Integrative Biology, University of South Florida, Tampa, FL 33620, USA.
出版信息
Appl Environ Microbiol. 2011 Jul;77(14):5050-5. doi: 10.1128/AEM.00130-11. Epub 2011 May 27.
Abstract
Environmental Enterococcus spp. were compared by BOX-PCR genotyping and 16S rRNA gene sequencing to clarify the predictive relationship of BOX-PCR fingerprints to species designation. BOX-PCR and 16S rRNA gene relationships agreed for 77% of strains. BOX-PCR provided superior intraspecies discrimination but incorrectly identified some strains to the species level and divided some species into multiple groups.
摘要
采用 BOX-PCR 基因分型和 16S rRNA 基因测序比较了环境肠球菌 spp.，以阐明 BOX-PCR 指纹图谱与种属鉴定的预测关系。BOX-PCR 和 16S rRNA 基因关系在 77%的菌株中一致。BOX-PCR 提供了更好的种内区分能力，但错误地将一些菌株鉴定到种的水平，并将一些种分为多个组。
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本文引用的文献
1
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J Appl Microbiol. 1998 Dec;85 Suppl 1:83S-89S. doi: 10.1111/j.1365-2672.1998.tb05286.x.
2
The importance of sediment and submerged aquatic vegetation as potential habitats for persistent strains of enterococci in a subtropical watershed.沉积物和淹没水生植被作为亚热带流域中持久性肠球菌菌株潜在栖息地的重要性。
Water Res. 2010 Dec;44(20):5857-66. doi: 10.1016/j.watres.2010.07.005. Epub 2010 Aug 2.
3
Evaluation of the enterococci indicator in biosolids using culture-based and quantitative PCR assays.使用基于培养和定量PCR检测法评估生物固体中的肠球菌指标。
Water Res. 2009 Nov;43(19):4878-87. doi: 10.1016/j.watres.2009.09.016. Epub 2009 Sep 11.
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5
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10
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J Appl Microbiol. 2006 Jun;100(6):1272-81. doi: 10.1111/j.1365-2672.2006.02879.x.
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