Department of Radiology, Kanazawa University Graduate School of Medical Science, Kanazawa, 920-8640, Japan.
Eur Radiol. 2011 Oct;21(10):2056-66. doi: 10.1007/s00330-011-2165-8. Epub 2011 May 29.
To clarify the changes in organic anion-transporting polypeptide 8 (OATP8) expression and enhancement ratio on gadoxetic acid-enhanced MR imaging in hepatocellular nodules during multistep hepatocarcinogenesis.
In imaging analysis, we focused on 71 surgically resected hepatocellular carcinomas (well, moderately and poorly differentiated HCCs) and 1 dysplastic nodule (DN). We examined the enhancement ratio in the hepatobiliary phase of gadoxetic acid enhanced MR imaging [(1/postcontrast T1 value-1/precontrast T1 value)/(1/precontrast T1 value)], then analysed the correlation among the enhancement ratio, tumour differentiation grade and intensity of immunohistochemical OATP8 expression. In pathological analysis, we focused on surgically resected 190 hepatocellular nodules: low-grade DNs, high-grade DNs, early HCCs, well-differentiated, moderately differentiated and poorly differentiated HCCs, including cases without gadoxetic acid-enhanced MR imaging. We evaluated the correlation between the immunohistochemical OATP8 expression and the tumour differentiation grade.
The enhancement ratio of HCCs decreased in accordance with the decline in tumour differentiation (P < 0.0001, R = 0.28) and with the decline of OATP8 expression (P < 0.0001, R = 0.81). The immunohistochemical OATP8 expression decreased from low-grade DNs to poorly differentiated HCCs (P < 0.0001, R = 0.15).
The immunohistochemical expression of OATP8 significantly decreases during multistep hepatocarcinogenesis, which may explain the decrease in enhancement ratio on gadoxetic acid-enhanced MR imaging.
阐明多步肝细胞癌发生过程中，有机阴离子转运多肽 8（OATP8）表达和增强比值在钆塞酸增强磁共振成像肝结节中的变化。
在影像学分析中，我们重点关注 71 个手术切除的肝细胞癌（高、中、低分化 HCC）和 1 个异型增生结节（DN）。我们检查了钆塞酸增强磁共振成像肝胆期的增强比值[(1/增强后 T1 值-1/增强前 T1 值)/(1/增强前 T1 值)]，然后分析了增强比值与肿瘤分化程度和 OATP8 免疫组化表达强度之间的相关性。在病理学分析中，我们重点关注手术切除的 190 个肝细胞结节：低级别 DN、高级别 DN、早期 HCC、高分化、中分化和低分化 HCC，包括无钆塞酸增强磁共振成像的病例。我们评估了 OATP8 免疫组化表达与肿瘤分化程度之间的相关性。
HCC 的增强比值随着肿瘤分化程度的降低而降低（P<0.0001，R=0.28），并且随着 OATP8 表达的降低而降低（P<0.0001，R=0.81）。OATP8 免疫组化表达从低级别 DN 降低到低分化 HCC（P<0.0001，R=0.15）。
OATP8 的免疫组化表达在多步肝细胞癌发生过程中显著降低，这可能解释了在钆塞酸增强磁共振成像中增强比值的降低。
