医院重症监护病房中人类共生菌中 1 类整合子的遗传背景和结构多样性。
Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit.
机构信息
The iThree Institute, University of Technology, P.O. Box 123, Broadway, Sydney, NSW 2007, Australia.
出版信息
Antimicrob Agents Chemother. 2011 Aug;55(8):3939-43. doi: 10.1128/AAC.01831-10. Epub 2011 May 31.
Abstract
Most surveys for class 1 integrons are at least partly predicated on PCR screening that targets integron conserved regions. However, class 1 integrons are structurally diverse, so dependence on conserved regions may lead to missing clinically relevant examples of class 1 integrons. Here, we surveyed a commensal population of bacteria from patients in an intensive care unit to identify class 1 integrons irrespective of their structure or genetic context. We identified several examples of class 1 integrons linked to complete Tn402-like or Tn402 hybrid transposition modules and diverse insertion points with respect to the inverted repeat IRi boundary. The diversity and abundance of class 1 integrons identified are such that many novel elements seen here would not have been identified by commonly used methods, and they revealed an additional level of complexity.
摘要
大多数针对 I 类整合子的调查至少部分基于针对整合子保守区域的 PCR 筛选。然而，I 类整合子结构多样，因此依赖保守区域可能导致错过临床上相关的 I 类整合子实例。在这里，我们调查了重症监护病房患者共生细菌群，以鉴定 I 类整合子，而不考虑其结构或遗传背景。我们鉴定了几个与完整 Tn402 样或 Tn402 混合转座模块以及与反向重复 IRi 边界有关的不同插入点相连的 I 类整合子的例子。鉴定的 I 类整合子的多样性和丰度如此之高，以至于这里看到的许多新元件都不会被常用方法所识别，并且它们揭示了额外的复杂性。
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2
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PLoS One. 2010 Sep 15;5(9):e12754. doi: 10.1371/journal.pone.0012754.
3
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Microb Drug Resist. 2010 Sep;16(3):197-202. doi: 10.1089/mdr.2010.0042.
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J Infect Dev Ctries. 2010 Jun 30;4(6):412-6.
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