Libisch Balázs, Abdulkadir Sahabi, Keresztény Tibor, Papp Péter P, Olasz Ferenc, Fébel Hedvig, Sándor Zsuzsanna J, Rasschaert Geertrui, Lambrecht Ellen, Heyndrickx Marc, Szabó András, Kovács Melinda, Posta Katalin
Agribiotechnology and Precision Breeding for Food Security National Laboratory, Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, 2100 Gödöllő, Hungary.
Bawu Veterinary Consult, Jalingo 660213, Nigeria.
Antibiotics (Basel). 2022 Oct 20;11(10):1441. doi: 10.3390/antibiotics11101441.
The aim of this study was metagenomics analyses of acquired antibiotic-resistance genes (ARGs) in the intestinal microbiome of two important food-animal species in Hungary from a One Health perspective. Intestinal content samples were collected from 12 domestic pigs () and from a common carp (). Shotgun metagenomic sequencing of DNA purified from the intestinal samples was performed on the Illumina platform. The ResFinder database was applied for detecting acquired ARGs in the assembled metagenomic contigs. Altogether, 59 acquired ARG types were identified, 51 genes from domestic pig and 12 genes from the carp intestinal microbiome. ARG types belonged to the antibiotic classes aminoglycosides (27.1%), tetracyclines (25.4%), β-lactams (16.9%), and others. Of the identified ARGs, (E), a - β-lactamase gene, as well as , , , , and , were identified only in carp but not in swine samples. Several of the detected acquired ARGs have not yet been described from food animals in Hungary. The (Q), (W), (O), and (A) genes detected in the intestinal microbiome of domestic pigs had also been identified from free-living wild boars in Hungary, suggesting a possible relationship between the occurrence of acquired ARGs in domestic and wild animal populations.
本研究的目的是从“同一个健康”的角度，对匈牙利两种重要食用动物肠道微生物群中获得性抗生素耐药基因（ARGs）进行宏基因组学分析。从12头家猪（ ）和1条鲤鱼（ ）采集肠道内容物样本。对从肠道样本中纯化的DNA进行鸟枪法宏基因组测序，测序在Illumina平台上进行。使用ResFinder数据库检测组装后的宏基因组重叠群中的获得性ARGs。共鉴定出59种获得性ARG类型，其中51种基因来自家猪肠道微生物群，12种基因来自鲤鱼肠道微生物群。ARG类型属于氨基糖苷类（27.1%）、四环素类（25.4%）、β-内酰胺类（16.9%）等抗生素类别。在鉴定出的ARGs中，（E），一种β-内酰胺酶基因，以及 、 、 、 和 仅在鲤鱼中鉴定到，在家猪样本中未鉴定到。在匈牙利，几种检测到的获得性ARGs尚未在家畜中被描述过。在家猪肠道微生物群中检测到的（Q）、（W）、（O）和（A）基因也在匈牙利自由生活的野猪中被鉴定到，这表明家畜和野生动物群体中获得性ARGs的出现可能存在关联。
