质谱分析完整蛋白质异构体。
Analysis of intact protein isoforms by mass spectrometry.
机构信息
Departmen of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.
出版信息
J Biol Chem. 2011 Jul 22;286(29):25451-8. doi: 10.1074/jbc.R111.239442. Epub 2011 Jun 1.
Abstract
The diverse proteome of an organism arises from such events as single nucleotide substitutions at the DNA level, different RNA processing, and dynamic enzymatic post-translational modifications. This minireview focuses on the measurement of intact proteins to describe the diversity found in proteomes. The field of biological mass spectrometry has steadily advanced, enabling improvements in the characterization of single proteins to proteins derived from cells or tissues. In this minireview, we discuss the basic technology for "top-down" intact protein analysis. Furthermore, examples of studies involved with the qualitative and quantitative analysis of full-length polypeptides are provided.
摘要
生物体的多样化蛋白质组来源于 DNA 水平的单核苷酸替换、不同的 RNA 加工以及动态酶翻译后修饰等事件。本篇综述重点关注完整蛋白质的测量，以描述蛋白质组中发现的多样性。生物质谱领域的技术不断进步，使对单个蛋白质的特性鉴定以及从细胞或组织中提取的蛋白质的特性鉴定都得到了改善。在本篇综述中，我们讨论了“自上而下”完整蛋白质分析的基本技术。此外，还提供了涉及全长多肽的定性和定量分析的研究示例。
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