应用改良 PCR 核糖体分型技术直接检测粪便样本中的艰难梭菌核糖体分型。
Use of modified PCR ribotyping for direct detection of Clostridium difficile ribotypes in stool samples.
机构信息
Institute of Public Health Maribor, Prvomajska 1, 2000 Maribor, Slovenia.
出版信息
J Clin Microbiol. 2011 Aug;49(8):3024-5. doi: 10.1128/JCM.01013-11. Epub 2011 Jun 1.
Abstract
PCR ribotyping was modified to allow direct detection of Clostridium difficile from stool samples. Direct PCR ribotyping was possible in 86 out of 99 C. difficile-positive stool samples, and in 84 cases (84.8%), the ribotype determined directly from the stool sample was identical to the ribotype of the strain isolated from the same stool sample.
摘要
聚合酶链反应核糖体分型技术被修改为可直接从粪便样本中检测艰难梭菌。在 99 份艰难梭菌阳性粪便样本中，有 86 份可直接进行聚合酶链反应核糖体分型，且在 84 例（84.8%）中，直接从粪便样本中确定的核糖体型与从同一粪便样本中分离的菌株的核糖体型完全相同。
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本文引用的文献
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Molecular typing methods for Clostridium difficile: pulsed-field gel electrophoresis and PCR ribotyping.艰难梭菌的分子分型方法：脉冲场凝胶电泳和聚合酶链反应核糖体分型
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Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.使用基于毛细管凝胶电泳的PCR核糖体分型法对艰难梭菌分离株进行鉴定。
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Coexistence of multiple PCR-ribotype strains of Clostridium difficile in faecal samples limits epidemiological studies.粪便样本中艰难梭菌多种PCR核糖体分型菌株的共存限制了流行病学研究。
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Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing.基于核糖体RNA基因测序开发一种用于艰难梭菌的新型PCR核糖体分型方法。
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