Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110012, India.
Mol Biol Rep. 2012 Feb;39(2):817-24. doi: 10.1007/s11033-011-0803-5. Epub 2011 Jun 2.
Plants have evolved diverse mechanism to recognize pathogen attack and triggers defense responses. These defense responses alter host cellular function regulated by endogenous, small, non-coding miRNAs. To understand the mechanism of miRNAs regulated cellular functions during stem rust infection in wheat, we investigated eight different miRNAs viz. miR159, miR164, miR167, miR171, miR444, miR408, miR1129 and miR1138, involved in three different independent cellular defense response to infection. The investigation reveals that at the initiation of disease, accumulation of miRNAs might be playing a key role in hypersensitive response (HR) from host, which diminishes at the maturation stage. This suggests a possible host-fungal synergistic relation leading to susceptibility. Differential expression of these miRNAs in presence and absence of R gene provides a probable explanation of miRNA regulated R gene mediated independent pathways.
植物已经进化出多种机制来识别病原体攻击并触发防御反应。这些防御反应改变了宿主细胞的功能，受内源性、小的、非编码的 miRNAs 调控。为了了解在小麦条锈菌感染过程中，miRNAs 调控细胞功能的机制，我们研究了参与三种不同独立细胞防御反应的 8 种不同的 miRNAs，miR159、miR164、miR167、miR171、miR444、miR408、miR1129 和 miR1138。研究表明，在疾病开始时，miRNAs 的积累可能在宿主的过敏反应（HR）中发挥关键作用，而在成熟阶段则减少。这表明可能存在一种宿主-真菌协同关系，导致易感性。在存在和不存在 R 基因的情况下，这些 miRNAs 的差异表达为 miRNA 调控 R 基因介导的独立途径提供了一个可能的解释。
