Department of Food Science and Nutrition, Zhejiang University , Hangzhou 310058, People's Republic of China.
J Agric Food Chem. 2011 Jul 13;59(13):7419-26. doi: 10.1021/jf2011722. Epub 2011 Jun 10.
8-Prenylnaringenin (8PN), which presents in hop, enjoys fame as the most potential phytoestrogen. Although a number of health effects are attributed to 8PN, few reports are available about the production of it. In this work, screening of fungi to efficiently transform isoxanthohumol (IXN) into 8PN was designed. The biotransformation of IXN was significantly observed in Eupenicillium javanicum, Cunninghamella blakesleana, and Ceriporiopsis subvermispora under five kinds of transformation conditions. As a comparative result of IXN transformation, E. javanicum was the optimal biocatalyst to produce 8PN. Transformation caused by growing precultured fungal mycelia, a process designated as G2, was a favorable condition for IXN transformation in view of the yield of 8PN. The possible transformation pathway of 8PN bioproduction is postulated in this work. The construction of fungus and transformation mode derived from the current work is viable and an alternative procedure for 8PN formation.
8- prenylnaringenin (8PN)，存在于啤酒花中，作为最有潜力的植物雌激素而闻名。尽管有许多健康效应归因于 8PN，但关于它的生产的报道很少。在这项工作中，设计了筛选真菌以有效转化异黄腐醇（IXN）为 8PN 的方法。在五种转化条件下，在 Eupenicillium javanicum、Cunninghamella blakesleana 和 Ceriporiopsis subvermispora 中，明显观察到 IXN 的生物转化。作为 IXN 转化的比较结果，E. javanicum 是生产 8PN 的最佳生物催化剂。通过生长预先培养的真菌菌丝的转化，即 G2 过程，是有利于 IXN 转化的条件，因为 8PN 的产量较高。本文提出了 8PN 生物生产的可能转化途径。从当前工作中得出的真菌构建和转化模式是可行的，是形成 8PN 的替代方法。
