Department of Molecular Biology and Microbiology, AECS, Damascus Syria.
Genet Mol Biol. 2010 Apr;33(2):328-32. doi: 10.1590/S1415-47572010005000041. Epub 2010 Jun 1.
The usefulness of IRAP (inter-retrotransposon amplified polymorphism) and ITS-RFLP (restriction of PCR-amplified internal transcribed spacers of the rDNA) markers in the analysis of 39 Pyrenophora graminea isolates was determined. Each marker system could discriminate between all of the isolates in detecting polymorphism, albeit with variable efficiency. IRAP and ITS-RFLP produced 85% and 77% polymorphic bands, respectively, with a corresponding mean polymorphic information content (PIC) of 0.38 and 0.36. The IRAP marker index ratio (2.41) was higher than ITS-RFLP (1.50). On one hand, the quality nature of data (QND) was higher for ITS-RFLP (0.169) than IRAP (0.093). However, correlation between both marker similarity matrices was significant (r = 0.34, p < 0.05). These findings suggest their combined use in phylogenetic analysis. To our knowledge, this is the first report of a comparison involving these two advanced DNA marker systems.
测定了间反转录转座子扩增多态性（IRAP）和 ITS-RFLP（PCR 扩增的 rDNA 内部转录间隔区的限制）标记在 39 个禾谷丝核菌分离物分析中的有用性。每个标记系统在检测多态性方面都可以区分所有分离物，尽管效率不同。IRAP 和 ITS-RFLP 分别产生 85%和 77%的多态性带，相应的平均多态信息含量（PIC）分别为 0.38 和 0.36。IRAP 标记指数比（2.41）高于 ITS-RFLP（1.50）。一方面，ITS-RFLP（0.169）的质量性质数据（QND）高于 IRAP（0.093）。然而，两种标记相似矩阵之间的相关性显著（r=0.34，p<0.05）。这些发现表明它们可在系统发育分析中联合使用。据我们所知，这是首次比较这两种先进 DNA 标记系统的报道。
