Departamento de Biologia Geral, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR Brazil.
Genet Mol Biol. 2010 Jan;33(1):169-75. doi: 10.1590/S1415-47572010005000006. Epub 2010 Mar 1.
Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types, plant (Allium cepa) and mammal (HTC) cells, for more accurately assessing genotoxicity in environmental samples.
多环芳烃（PAHs）是在炼油厂废水中常见的遗传毒性化学物质。可以使用植物和细胞培养物的生物测定法来评估环境安全性和潜在的遗传毒性。在这项研究中，通过洋葱根尖微核（MN）试验分析了炼油厂废水的遗传毒性潜力，结果表明在 24 小时的处理后没有效果。另一方面，通过彗星试验仅在暴露 2 小时后，就观察到了大鼠（Rattus norvegicus）肝癌细胞（HTC）中 DNA 的原发性损伤。考虑到检测不同性质的 DNA 损伤的能力以及这些细胞对外源化学物质的代谢能力，我们建议将这两种生物测定法与植物（洋葱）和哺乳动物（HTC）细胞这两种细胞类型相结合，以更准确地评估环境样本中的遗传毒性。
