Marine Bioresources and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources of China, Qingdao 266061, People's Republic of China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, People's Republic of China.
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China.
Ecotoxicol Environ Saf. 2021 Jan 15;208:111655. doi: 10.1016/j.ecoenv.2020.111655. Epub 2020 Nov 20.
An enormous amount of oil-containing drill cuttings have been produced by the marine oil and gas industry. The environmental impacts of discharged drilling waste have been extensively studied. However, there is still an urgent need to develop alternative methods to identify the genotoxicity of untreated and treated drill waste in a timely manner before it is discharged. In this study, we developed a relatively rapid, sensitive, and accurate genotoxicity-detection method using Comet assay and the marine benthic goby Mugilogobius chulae. This goby is sensitive to a standard toxicant mitomycin C (MMC). The optimal exposure period for genotoxicity detection using M. chulae was determined. Three genotoxic indices (tail length (TL), tail DNA content (TD), and tail moment (TM)) were used to assess the effectiveness of high-temperature treatment of oil-contaminated waste. Untreated oil-containing drill cuttings exhibited the highest genotoxicity to goby cells. Genotoxicity was dramatically reduced after thermal treatment of drill cuttings at 350 °C and 500 °C. TD and TM exhibited significant correlation with the concentration of total petroleum hydrocarbons (TPHs)/total polycyclic aromatic hydrocarbons (PAHs) according to Pearson and Mantel correlation analyses (P values were <0.05). Using redundancy analysis (RDA) and variation partition analysis (VPA), the genotoxic effects of the drill cuttings were ascribed to total alkanes and specific groups of PAHs. In conclusion, this newly established biological model has the potential to be widely used to detect the genetic damage of untreated or treated oil-containing drill cuttings discharged into the marine environment.
海洋油气行业产生了大量含油钻屑。已广泛研究了排放钻井废物的环境影响。然而，仍然迫切需要开发替代方法，以便在排放未经处理和处理的钻屑之前及时识别其遗传毒性。在这项研究中，我们使用彗星分析和海洋底栖虾虎鱼 Mugilogobius chulae 开发了一种相对快速、敏感和准确的遗传毒性检测方法。这种虾虎鱼对标准毒物丝裂霉素 C（MMC）敏感。确定了使用 M. chulae 进行遗传毒性检测的最佳暴露期。三个遗传毒性指数（尾长（TL）、尾 DNA 含量（TD）和尾矩（TM））用于评估高温处理含油废物的效果。未经处理的含油钻屑对虾虎鱼细胞表现出最高的遗传毒性。在 350°C 和 500°C 下对钻屑进行热处理后，遗传毒性大大降低。TD 和 TM 与总石油烃（TPHs）/总多环芳烃（PAHs）浓度之间呈显著相关性，根据 Pearson 和 Mantel 相关分析（P 值<0.05）。使用冗余分析（RDA）和变分分解分析（VPA），将钻屑的遗传毒性归因于总烷烃和特定组的 PAHs。总之，这种新建立的生物模型有可能广泛用于检测排放到海洋环境中的未经处理或处理的含油钻屑的遗传损伤。
