钙离子和二价阳离子载体A23187对仙台病毒引起鸡红细胞凝集和融合的影响。
Effects of calcium ions and the bivalent cation ionophore A23187 on the agglutination and fusion of chicken erythrocytes by Sendai virus.
作者信息
Hart C A, Fisher D, Hallinan T, Lucy J A
出版信息
Biochem J. 1976 Jul 15;158(1):141-5. doi: 10.1042/bj1580141.
Abstract
- Ca2+ (0.4-16 mM) had no detectable action on the agglutination of hen erythrocytes by Sendai virus. 2. Pretreatment of the cells with Ca2+ (0.1-8 mM) in the presence of the bivalent cation ionophore A23187 led, however, to a significant decrease in the subsequent agglutination of the cells by the virus. 3. It thus appears that the entry of Ca2+ into the interior of these cells decreases cellular agglutination by Sendai virus; possible interpretations of this phenomenon are discussed in terms of the movement of intramembranous particles. 4. With a small number of virions, maximum cell fusion by Sendai virus occurred in the presence of EGTA [ethanedioxybis(ethylamine)tetra-acetate]. 5. Virus-induced cell fusion was significantly decreased by Ca2+, even at a concentration of 0.2 mM; it is suggested that this may result from diminished interactions between virus particles and erythrocyte membranes.
摘要
- 钙离子（0.4 - 16毫摩尔）对仙台病毒引起的鸡红细胞凝集没有可检测到的作用。2. 然而，在二价阳离子载体A23187存在的情况下，用钙离子（0.1 - 8毫摩尔）预处理细胞，会导致随后病毒对细胞的凝集显著减少。3. 因此，似乎钙离子进入这些细胞内部会减少仙台病毒引起的细胞凝集；根据膜内颗粒的移动对这一现象进行了可能的解释。4. 对于少量病毒粒子，在乙二醇双（乙胺）四乙酸（EGTA）存在的情况下，仙台病毒会发生最大程度的细胞融合。5. 即使钙离子浓度为0.2毫摩尔，病毒诱导的细胞融合也会显著减少；这表明这可能是由于病毒粒子与红细胞膜之间的相互作用减弱所致。
相似文献
1
Effects of calcium ions and the bivalent cation ionophore A23187 on the agglutination and fusion of chicken erythrocytes by Sendai virus.钙离子和二价阳离子载体A23187对仙台病毒引起鸡红细胞凝集和融合的影响。
Biochem J. 1976 Jul 15;158(1):141-5. doi: 10.1042/bj1580141.
2
Changes in the distribution of intramembranous particles in hen erythrocytes during cell fusion induced by the bivalent-cation ionophore A23187.在二价阳离子离子载体A23187诱导的细胞融合过程中，母鸡红细胞膜内颗粒分布的变化
Biochem J. 1976 Sep 15;158(3):651-3. doi: 10.1042/bj1580651.
3
The effect of intracellular calcium ions on adrenaline-stimulated adenosine 3':5'-cyclic monophosphate concentrations in pigeon erythrocytes, studied by using the ionophore A23187.通过使用离子载体A23187研究细胞内钙离子对鸽红细胞中肾上腺素刺激的3':5'-环磷酸腺苷浓度的影响。
Biochem J. 1976 Aug 15;158(2):211-21. doi: 10.1042/bj1580211.
4
Production of 1,2-diacylglycerol and phosphatidate in human erythrocytes treated with calcium ions and ionophore A23187.钙离子和离子载体A23187处理的人红细胞中1,2 - 二酰甘油和磷脂酸的产生
Biochem J. 1976 May 15;156(2):225-32. doi: 10.1042/bj1560225.
5
Calcium ions and adenosine 3':5'-cyclic monophosphate in hepatocytes: effects of ethanedioxybis(ethylamine)tetra-acetate (EGTA) and ionophore A23187 [proceedings].肝细胞中的钙离子与3':5'-环磷酸腺苷：乙二氧基双（乙胺）四乙酸（EGTA）和离子载体A23187的作用[会议论文集]
Biochem Soc Trans. 1979 Oct;7(5):1032-3. doi: 10.1042/bst0071032.
6
Inhibition of membrane fusion by suppression of lateral movement of membrane proteins.通过抑制膜蛋白的侧向运动来抑制膜融合。
Biochim Biophys Acta. 1978 Dec 19;514(2):213-24. doi: 10.1016/0005-2736(78)90293-6.
7
Calcium and lipolysis in adipose tissue: effects of ethanedioxybis-(ethylamine)tetra-acetate (EGTA) and ionophore A23187 [proceedings].
Biochem Soc Trans. 1977;5(4):959-62. doi: 10.1042/bst0050959.
8
Calcium-dependent stimulation of erythrocyte membrane phospholipid fatty acid incorporation by the ionophore A23187.离子载体A23187对红细胞膜磷脂脂肪酸掺入的钙依赖性刺激作用。
J Biol Chem. 1976 Jul 10;251(13):4162-4.
9
Microvesiculation and sphingomyelinase activation in chicken erythrocytes treated with ionophore A23187 and Ca2+.用离子载体A23187和Ca2+处理的鸡红细胞中的微囊泡形成和鞘磷脂酶激活
Biochim Biophys Acta. 1982 Dec 8;693(1):53-67. doi: 10.1016/0005-2736(82)90470-9.
10
Role of Ca++ in virus-induced membrane fusion. Ca++ accumulation and ultrastructural changes induced by Sendai virus in chicken erythrocytes.钙离子在病毒诱导的膜融合中的作用。仙台病毒在鸡红细胞中诱导的钙离子积累和超微结构变化。
J Cell Biol. 1978 Aug;78(2):465-79. doi: 10.1083/jcb.78.2.465.
引用本文的文献
1
Chlorotetracycline induces calcium mediated shape changes in human erythrocytes. Is Ca asymmetrically distributed in the red cell membrane?金霉素诱导人红细胞中钙介导的形态变化。钙在红细胞膜中是否呈不对称分布？
Pflugers Arch. 1977;372(3):259-68. doi: 10.1007/BF01063861.
2
Role of Ca++ in virus-induced membrane fusion. Ca++ accumulation and ultrastructural changes induced by Sendai virus in chicken erythrocytes.钙离子在病毒诱导的膜融合中的作用。仙台病毒在鸡红细胞中诱导的钙离子积累和超微结构变化。
J Cell Biol. 1978 Aug;78(2):465-79. doi: 10.1083/jcb.78.2.465.
3
Membrane cholesterol and cell fusion of hen and guinea-pig erythrocytes.母鸡和豚鼠红细胞的膜胆固醇与细胞融合
Biochem J. 1977 Aug 15;166(2):255-63. doi: 10.1042/bj1660255.
本文引用的文献
1
2
3
ELECTRONIC PARTICLE COUNTING APPLIED TO THE QUANTITATIVE STUDY OF RED CELL AGGLUTINATION.电子粒子计数法在红细胞凝集定量研究中的应用
Transfusion. 1964 May-Jun;4:153-68. doi: 10.1111/j.1537-2995.1964.tb02852.x.
4
Control of myogenesis in vitro by Ca 2 + concentration in nutritional medium.营养培养基中钙离子浓度对体外成肌作用的控制
Exp Cell Res. 1969 Nov;58(1):163-7. doi: 10.1016/0014-4827(69)90127-x.
5
The structure of erythrocyte membranes studied by freeze-etching. II. Localization of receptors for phytohemagglutinin and influenza virus to the intramembranous particles.通过冷冻蚀刻研究红细胞膜的结构。II. 植物血凝素和流感病毒受体在膜内颗粒上的定位。
J Exp Med. 1972 Jun 1;135(6):1209-27. doi: 10.1084/jem.135.6.1209.
6
A quantitative assay for measuring cell agglutination: agglutination of sea urchin embryo and mouse teratoma cells by concanavalin A.一种用于测量细胞凝集的定量测定法：伴刀豆球蛋白A对海胆胚胎和小鼠畸胎瘤细胞的凝集作用
Exp Cell Res. 1972 Aug;73(2):475-80. doi: 10.1016/0014-4827(72)90074-2.
7
A microfluorimetric study of translational diffusion in erythrocyte membranes.红细胞膜中平移扩散的微量荧光测定研究。
Biochim Biophys Acta. 1974 Nov 15;367(3):282-94. doi: 10.1016/0005-2736(74)90085-6.
8
The biochemistry of virus-induced cell fusion. Changes in membrane integrity.病毒诱导的细胞融合的生物化学。膜完整性的变化。
Biochem J. 1974 Jun;140(3):405-11. doi: 10.1042/bj1400405.
9
Fusion of intact human erythrocytes and erythrocyte ghosts.完整人类红细胞与红细胞血影的融合。
J Cell Biol. 1974 Oct;63(1):1-11. doi: 10.1083/jcb.63.1.1.
10
Characteristics of Sendai virus receptors in a model membrane.模型膜中仙台病毒受体的特征
J Mol Biol. 1974 Mar 15;83(4):427-36. doi: 10.1016/0022-2836(74)90504-x.
Zotero 插件