Department of Bioengineering, Rice University, 6100 Main Street, Houston, Texas 77005, USA.
J Biomed Opt. 2011 May;16(5):056005. doi: 10.1117/1.3574756.
Hyperspectral imaging has tremendous potential to detect important molecular biomarkers of early cancer based on their unique spectral signatures. Several drawbacks have limited its use for in vivo screening applications: most notably the poor temporal and spatial resolution, high expense, and low optical throughput of existing hyperspectral imagers. We present the development of a new real-time hyperspectral endoscope (called the image mapping spectroscopy endoscope) based on an image mapping technique capable of addressing these challenges. The parallel high throughput nature of this technique enables the device to operate at frame rates of 5.2 frames per second while collecting a (x, y, λ) datacube of 350 × 350 × 48. We have successfully imaged tissue in vivo, resolving a vasculature pattern of the lower lip while simultaneously detecting oxy-hemoglobin.
基于独特的光谱特征，高光谱成象技术在探测早期癌症的重要分子生物标志物方面具有巨大潜力。但由于现有高光谱成像仪存在时间和空间分辨率差、成本高、光学效率低等缺点，其在体内筛选中的应用受到限制。我们介绍了一种新的实时高光谱内窥镜（称为图像映射光谱内窥镜）的开发，该内窥镜基于一种图像映射技术，能够解决这些挑战。该技术的并行高通量特性使该设备能够以每秒 5.2 帧的速度运行，同时采集 350×350×48 的 (x,y,λ) 数据立方体。我们已经成功地对体内组织进行了成像，同时检测到氧合血红蛋白，解析了下唇的血管模式。
