Modir Naeeme, Shahedi Maysam, Dormer James, Ma Ling, Fei Baowei
University of Texas at Dallas, Department of Bioengineering, Richardson, Texas, United States.
University of Texas at Dallas, Center for Imaging and Surgical Innovation, Richardson, Texas, United States.
J Med Imaging (Bellingham). 2025 May;12(3):035002. doi: 10.1117/1.JMI.12.3.035002. Epub 2025 Jun 12.
This study demonstrates the feasibility of using an LED array for hyperspectral imaging (HSI). The prototype validates the concept and provides insights into the design of future HSI applications. Our goal is to design, develop, and test a real-time, LED-based HSI prototype as a proof-of-principle device for hyperspectral imaging using LEDs.
A prototype was designed based on a multiwavelength LED array and a monochrome camera and was tested to investigate the properties of the LED-based HSI. The LED array consisted of 18 LEDs in 18 different wavelengths from 405 nm to 910 nm. The performance of the imaging system was evaluated on different normal and cancerous tissues. The impact of imaging conditions on the HSI quality was investigated. The LED-based HSI device was compared with a reference hyperspectral camera system.
The hyperspectral signatures of different imaging targets were acquired using our prototype HSI device, which are comparable to the data obtained using the reference HSI system.
The feasibility of employing a spectral LED array as the illumination source for high-speed and high-quality HSI has been demonstrated. The use of LEDs for HSI can open the door to numerous applications in endoscopic, laparoscopic, and handheld HSI devices.
本研究证明了使用发光二极管（LED）阵列进行高光谱成像（HSI）的可行性。该原型验证了这一概念，并为未来高光谱成像应用的设计提供了见解。我们的目标是设计、开发并测试一款基于LED的实时高光谱成像原型，作为使用发光二极管进行高光谱成像的原理验证设备。
基于多波长发光二极管阵列和单色相机设计了一个原型，并对其进行测试以研究基于发光二极管的高光谱成像的特性。该发光二极管阵列由18个发光二极管组成，波长范围从405纳米至910纳米，共18种不同波长。成像系统的性能在不同的正常组织和癌组织上进行了评估。研究了成像条件对高光谱成像质量的影响。将基于发光二极管的高光谱成像设备与参考高光谱相机系统进行了比较。
使用我们的高光谱成像原型设备获取了不同成像目标的高光谱特征，这些特征与使用参考高光谱成像系统获得的数据相当。
已证明采用光谱发光二极管阵列作为高速、高质量高光谱成像照明源的可行性。将发光二极管用于高光谱成像可为内窥镜、腹腔镜和手持式高光谱成像设备的众多应用打开大门。
