Sheffield University, Department of Molecular Biology and Biotechnology, Western Bank, Sheffield S10 2TN, United Kingdom.
Fungal Biol. 2011 Jun;115(6):547-56. doi: 10.1016/j.funbio.2011.02.001. Epub 2011 Feb 24.
Fungi can grow in a variety of growth forms: yeast, pseudohyphae and hyphae. The human fungal pathogen Candida albicans can grow in all three of these forms. In this fungus, hyphal growth is distinguished by the presence of a Spitzenkörper-like structure at the hyphal tip and a band of septin bars around the base of newly evaginated germ tubes. The budding yeast Saccharomyces cerevisiae grows as yeast and pseudohyphae, but is not normally considered to show hyphal growth. We show here that in mating projections of both C. albicans and S. cerevisiae a Spitzenkörper-like structure is present at the growing tip and a band of septin bars is present at the base. Furthermore, in S. cerevisiae mating projections, Spa2 and Bni1 form a cap to the 3-dimensional ball of FM4-64 staining, exactly as previously observed in C. albicans hyphae, suggesting that the putative Spitzenkörper may be a distinct structure from the polarisome. Taken together this work shows that mating projections of both S. cerevisiae and C. albicans show the key characteristics of hyphal growth.
酵母、假菌丝和菌丝。人类真菌病原体白色念珠菌可以在这三种形式中生长。在这种真菌中，菌丝生长的特征是在菌丝顶端存在类似于 Spitzenkörper 的结构，以及在新出芽的芽管基部存在一圈隔膜带。出芽酵母酿酒酵母可以作为酵母和假菌丝生长，但通常不被认为表现出菌丝生长。我们在这里表明，在白色念珠菌和酿酒酵母的交配突起中，在生长尖端存在类似于 Spitzenkörper 的结构，在基部存在隔膜带。此外，在酿酒酵母交配突起中，Spa2 和 Bni1 形成了 FM4-64 染色的三维球体的帽状结构，与之前在白色念珠菌菌丝中观察到的完全一致，这表明假定的 Spitzenkörper 可能与极体是不同的结构。总之，这项工作表明，酿酒酵母和白色念珠菌的交配突起都表现出菌丝生长的关键特征。
