Department of Chemistry and Center of Advanced Studies in Chemistry, Panjab University, Chandigarh, India.
Colloids Surf B Biointerfaces. 2011 Oct 1;87(1):173-9. doi: 10.1016/j.colsurfb.2011.05.018. Epub 2011 May 13.
The highly stable innocuous niosomes composed of four components (Triton X 100, polyethylene glycol 2000, water, Span 80) have been prepared successfully and characterized using particle size analyzer, transmission and scanning electron microscopy. The mean size has been found to be in the range 200-300 nm. The optimization of niosomes has been carried out using fluorescence spectroscopy. An attempt has been made to incorporate anti-tuberculosis drugs (ATD's) in the prepared niosomes. The stability and encapsulation efficiency of these drugs in the niosome have also been assessed and high encapsulation efficiency is observed. Such high encapsulation efficiency will serve as an advantage to solve the problem of multi-drug resistance in case of tuberculosis. Release studies and kinetics have been carried out to investigate the release behavior of drugs from the prepared niosomes. Fickian or diffusional release has been observed for rifampicin and isoniazid and a non-Fickian release mechanism for pyrazinamide. Fluorescence probe quenching technique has been used to determine the location and distribution coefficient of the ATD's in niosome/water system.
已成功制备并采用粒径分析仪、透射电子显微镜和扫描电子显微镜对由四种成分（Triton X-100、聚乙二醇 2000、水、Span 80）组成的高度稳定、无毒的非离子囊泡进行了表征。平均粒径范围在 200-300nm 之间。采用荧光光谱法对非离子囊泡进行了优化。尝试将抗结核药物（ATD）包封入制备的非离子囊泡中。还评估了这些药物在非离子囊泡中的稳定性和包封效率，观察到了高包封效率。这种高包封效率将有助于解决结核病多药耐药的问题。进行了释放研究和动力学研究，以研究药物从制备的非离子囊泡中的释放行为。利福平和异烟肼观察到菲克扩散释放，而吡嗪酰胺则观察到非菲克释放机制。荧光探针猝灭技术用于确定 ATD 在非离子囊泡/水体系中的位置和分配系数。
