通过纳米颗粒跟踪分析和扫描电子显微镜对人源细胞来源的外泌体进行表征。
Characterisation of exosomes derived from human cells by nanoparticle tracking analysis and scanning electron microscopy.
机构信息
Institute of Inorganic Chemistry, Faculty of Chemistry, University of Duisburg-Essen, Essen, Germany.
出版信息
Colloids Surf B Biointerfaces. 2011 Oct 1;87(1):146-50. doi: 10.1016/j.colsurfb.2011.05.013. Epub 2011 May 12.
Exosomes from three different cell types (HEK 293T, ECFC, MSC) were characterised by scanning electron microscopy (SEM), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The diameter was around 110 nm for the three cell types. The stability of exosomes was examined during storage at -20°C, 4°C, and 37°C. The size of the exosomes decreased at 4°C and 37°C, indicating a structural change or degradation. Multiple freezing to -20°C and thawing did not affect the exosome size. Multiple ultracentrifugation also did not change the exosome size.
通过扫描电子显微镜（SEM）、动态光散射（DLS）和纳米颗粒跟踪分析（NTA）对来自三种不同细胞类型（HEK 293T、ECFC、MSC）的外泌体进行了表征。这三种细胞类型的直径约为 110nm。在-20°C、4°C 和 37°C 下储存时，考察了外泌体的稳定性。外泌体在 4°C 和 37°C 下的尺寸减小，表明结构发生变化或降解。反复冻融至-20°C 并不影响外泌体的大小。多次超速离心也不会改变外泌体的大小。
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