比较研究人骨髓间充质干细胞和心肌细胞衍生外泌体对改善异丙肾上腺素诱导的缺血性损伤心肌细胞的作用。
Comparative study the effect of human bone marrow mesenchymal stem cells and cardiomyocytes derived exosomes on improvement of Isoproterenol induced ischemic damaged cardiomyocytes.
作者信息
Edalati Saeideh, Asadi Pouyan, Arabi Mehdi Sheikh, Khajeniazi Safoura
机构信息
Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
出版信息
J Stem Cells Regen Med. 2025 May 29;21(1):19-24. doi: 10.46582/jsrm.2101004. eCollection 2025.
BACKGROUND
Exosomes are small vesicles with intracellular origin which are released into the extracellular space. The properties of stem cell-derived exosomes are similar to their cellular origin and can be involved in repair the damaged tissues. This investigation aimed to evaluate the effect of exosomes derived from mesenchymal stem cells and cardiomyocytes on the repair of damaged cardiomyocyte in vitro.
METHODS
In this study, first damaged cells were created by Isopreternol treatment of mesenchymal-derived cardiomyocytes, then cells were affected by exosomes extracted from MSCs and MSC derived cardiomyocyes. Finally, mRNA levels of cardiac differentiation and damage markers were measured by Real Time PCR at the time intervals.
RESULTS
Our results showed the level of cardiac markers in damaged cardiomyocytes increased after treatment by cardiomyocytes derived exosomes compared to MSC derived exosomes. Reciprocally LDH a and b mRNA levels decrease in both conditions.
CONCLUSION
our findings revealed the exosomes extracted from cardiomyocytes were more effective in the repair of injured cells compared to the exosomes derived MSCs.
背景
外泌体是起源于细胞内的小囊泡，释放到细胞外空间。干细胞来源的外泌体的特性与其细胞起源相似，可参与受损组织的修复。本研究旨在评估间充质干细胞和心肌细胞来源的外泌体对体外受损心肌细胞修复的影响。
方法
在本研究中，首先通过异丙肾上腺素处理间充质来源的心肌细胞来创建受损细胞，然后细胞受到从间充质干细胞和间充质干细胞衍生的心肌细胞中提取的外泌体的影响。最后，在不同时间间隔通过实时聚合酶链反应测量心脏分化和损伤标志物的mRNA水平。
结果
我们的结果表明，与间充质干细胞来源的外泌体相比，心肌细胞来源的外泌体处理后受损心肌细胞中的心脏标志物水平升高。相反，在两种情况下乳酸脱氢酶a和b的mRNA水平均降低。
结论
我们的研究结果表明，与间充质干细胞来源的外泌体相比，从心肌细胞中提取的外泌体在修复受损细胞方面更有效。
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