Kasukurti A, Potcoava M, Desai S A, Eggleton C, Marr D W M
Chemical Engineering Department, Colorado School of Mines, Golden, CO 80401, USA.
Opt Express. 2011 May 23;19(11):10377-86. doi: 10.1364/OE.19.010377.
A low-cost single-cell isolation system incorporating a digital versatile disc burner (DVD RW) optical pickup has been developed. We show that these readily available modules have the required laser power and focusing optics to provide a steady Gaussian beam capable of optically trapping micron-sized colloids and red blood cells. Utility of the pickup is demonstrated through the non-destructive isolation of such particles in a laminar-flow based microfluidic device that captures and translates single microscale objects across streamlines into designated channel exits. In this, the integrated objective lens focusing coils are used to steer the optical trap across the channel, resulting in the isolation of colloids and red blood cells using a very inexpensive off-the-shelf optical component.
已开发出一种结合数字多功能光盘刻录机（DVD RW）光学头的低成本单细胞分离系统。我们表明，这些现成的模块具有所需的激光功率和聚焦光学元件，可提供稳定的高斯光束，能够对微米级胶体和红细胞进行光学捕获。通过在基于层流的微流控装置中对这些颗粒进行无损分离，证明了该光学头的实用性，该装置可捕获单个微观物体并将其跨流线平移到指定的通道出口。在此过程中，集成的物镜聚焦线圈用于引导光阱穿过通道，从而使用非常便宜的现成光学元件实现胶体和红细胞的分离。
