School of Electrical and Electronic Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Seberang Perai Selatan, Pulau Pinang, Malaysia. motasem
Talanta. 2011 Jul 15;85(1):28-34. doi: 10.1016/j.talanta.2011.04.069. Epub 2011 May 5.
Recent advances in microfluidic systems, particularly in the Micro Total Analysis System (μTAS) or Lab On a Chip (LOC), drive the current analysis tools and equipment towards miniaturization, rapid at-line testing and mobility. The state-of-the-art microfluidic technology targets a wider range but smaller volumes of analytes, making the analytical procedure relatively easier and faster. This trend together with faster electronics and modern instrumentation systems will make real-time and in situ analysis a definite possibility. This review focuses on microchip capillary electrophoresis with amperometric detection (MCE-AD) for the detection of DNA and other electroactive analytes. The problems associated with the microchip design, in particular the choice of materials and the configuration of electrodes are discussed thoroughly and solutions are proposed. Significant developments in the related areas are also covered and reviewed critically.
近年来，微流控系统领域，特别是在微全分析系统（μTAS）或芯片实验室（LOC）方面的进展，推动了当前分析工具和设备向小型化、在线快速检测和移动性方向发展。最先进的微流控技术针对更广泛但体积更小的分析物，使分析过程相对更容易和更快。这种趋势与更快的电子和现代仪器系统相结合，将使实时和现场分析成为一种确定的可能性。本综述重点介绍了用于检测 DNA 和其他电活性分析物的微芯片毛细管电泳与安培检测（MCE-AD）。本文深入讨论了与微芯片设计相关的问题，特别是材料的选择和电极的配置，并提出了解决方案。还涵盖并批判性地回顾了相关领域的重要发展。
