Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine (MOE 985 Innovative Platform), and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, P. R. China.
Talanta. 2011 Jul 15;85(1):136-41. doi: 10.1016/j.talanta.2011.03.035. Epub 2011 Mar 30.
This study presents the investigation of bioconjugating ability of near-infrared (NIR) CdSeTe/ZnS quantum dots (QDs) (710 nm) and visible CdSe QDs (595 nm) in immunofluorescent staining for cancer biomarkers in gastric cancer tissues probed with the homemade Hadamard transform (HT) spectral imaging microscope and a commercial multispectral imaging system. The results show that imunostaining ability of NIR QDs probes is stronger than that of visible QDs when the two kinds of QDs are simultaneously used to probe the cancer biomarkers such as cytokeratin 20 (CK20) and proliferating cell nuclear antigen (PCNA) in gastric cancer tissues. Moreover, when the two QDs probes are used for immunostaining successively for the same target molecules, staining order has great influences on the final results due to their different conjugating ability to the marker proteins. The results imply that NIR QDs hold more promise for real-time imaging of tumor tissues due to its higher sensitivity and contrast. In addition, the results also demonstrate the potential of Hadamard transform spectral imaging as a useful tool in biomedical analysis and quantitative evaluation for tumor tissues.
本研究探讨了近红外（NIR）CdSeTe/ZnS 量子点（QDs）（710nm）和可见 CdSe QDs（595nm）在胃癌组织中癌症生物标志物免疫荧光染色中的生物共轭能力，使用的是自制的 Hadamard 变换（HT）光谱成像显微镜和商业多光谱成像系统。结果表明，当两种 QDs 同时用于探测胃癌组织中的细胞角蛋白 20（CK20）和增殖细胞核抗原（PCNA）等癌症生物标志物时，NIR QDs 探针的免疫染色能力强于可见 QDs。此外，当两种 QDs 探针用于同一靶分子的连续免疫染色时，由于它们与标记蛋白的不同共轭能力，染色顺序对最终结果有很大影响。这些结果表明，由于具有更高的灵敏度和对比度，NIR QDs 更有望用于肿瘤组织的实时成像。此外，研究结果还证明了 Hadamard 变换光谱成像作为生物医学分析和肿瘤组织定量评估的有用工具的潜力。
