Neuro-oncology Program, Moores Cancer Center, University of California, San Diego, USA.
Bioconjug Chem. 2011 Aug 17;22(8):1638-44. doi: 10.1021/bc200201e. Epub 2011 Aug 1.
Quantum dots (QDs) are attracting intense interest as fluorescence labeling agents for biomedical imaging because biocompatible coatings and relatively nontoxic rare earth metal QDs have emerged as possible options. QD photoemissions are bright, of narrow wavelength range, and very stable. We sought to encapsulate QDs within targeted PEGylated liposomes to reduce their propensity for liver uptake and to amplify the already strong QD emission signal. A novel lipid-QD conjugate initialized a process by which lipids in solution coalesced around the QDs. The liposomal structure was confirmed with size measurements, SEM, and IR spectroscopy. PEGylated QD liposomes injected into a xenograft tumor model largely cleared from the body within 24 h. Residual liver labeling was low. Targeted QD liposomes exhibited robust tumor labeling compared with controls. This study highlights the potential of these near IR emitting QD liposomes for preclinical/clinical applications.
量子点 (QD) 作为荧光标记物在生物医学成像中引起了极大的兴趣，因为生物相容性涂层和相对无毒的稀土金属 QD 已经成为可能的选择。QD 的光发射亮度高、波长范围窄且非常稳定。我们试图将 QD 封装在靶向性聚乙二醇化脂质体中，以降低它们在肝脏中的摄取倾向，并放大已经很强的 QD 发射信号。一种新型脂质-QD 缀合物启动了一个过程，其中溶液中的脂质在 QD 周围凝聚。通过粒径测量、SEM 和 IR 光谱证实了脂质体的结构。注射入异种移植肿瘤模型的 PEG 化 QD 脂质体在 24 小时内大部分从体内清除。残留的肝脏标记物较少。与对照组相比，靶向 QD 脂质体表现出更强的肿瘤标记。这项研究强调了这些近红外发射 QD 脂质体在临床前/临床应用中的潜力。
