尿苷-5′-O-(2-硫代二磷酸)-N-乙酰葡萄糖胺的酶促合成及性质。
Enzymatic synthesis and properties of uridine-5'-O-(2-thiodiphospho)-N-acetylglucosamine.
机构信息
Departments of Chemistry and Biochemistry, The Ohio State University, 484 W. 12th Ave., Columbus, OH 43210, USA.
出版信息
Carbohydr Res. 2011 Sep 6;346(12):1576-80. doi: 10.1016/j.carres.2011.05.005. Epub 2011 May 12.
Abstract
This paper describes an enzymatic approach to obtain a thio-containing UDP-GlcNAc analog. We use an assay based on capture of the carbohydrate and analysis by mass spectrometry to quantitatively characterize the activity of this unnatural sugar donor in a LgtA-mediated glycosylation reaction.
摘要
本文描述了一种获得含硫 UDP-GlcNAc 类似物的酶法途径。我们使用基于糖捕获和质谱分析的测定方法，定量表征了这种非天然糖供体在 LgtA 介导的糖基化反应中的活性。
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