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1
Donor substrate promiscuity of bacterial β1-3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1-4-galactosyltransferases.细菌β1-3-N-乙酰葡糖胺基转移酶的供体底物混杂性及β1-4-半乳糖基转移酶的受体底物灵活性。
Bioorg Med Chem. 2016 Apr 15;24(8):1696-705. doi: 10.1016/j.bmc.2016.02.043. Epub 2016 Mar 3.
2
One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates.用于碳水化合物化学酶法合成的一锅多酶（OPME）系统。
Org Biomol Chem. 2016 Mar 14;14(10):2809-18. doi: 10.1039/c6ob00058d.
3
Human Milk Oligosaccharides (HMOS): Structure, Function, and Enzyme-Catalyzed Synthesis.人乳寡糖（HMOS）：结构、功能及酶催化合成
Adv Carbohydr Chem Biochem. 2015;72:113-90. doi: 10.1016/bs.accb.2015.08.002. Epub 2015 Nov 11.
4
Sequential one-pot multienzyme (OPME) synthesis of lacto-N-neotetraose and its sialyl and fucosyl derivatives.乳糖 - N - 新四糖及其唾液酸化和岩藻糖基化衍生物的顺序一锅多酶（OPME）合成。
Chem Commun (Camb). 2015 May 4;51(36):7689-92. doi: 10.1039/c5cc01330e.
5
4-O-Acetyl-sialic acid (Neu4,5Ac2) in acidic milk oligosaccharides of the platypus (Ornithorhynchus anatinus) and its evolutionary significance.鸭嘴兽（Ornithorhynchus anatinus）酸性乳寡糖中的4-O-乙酰神经氨酸（Neu4,5Ac2）及其进化意义。
Glycobiology. 2015 Jun;25(6):683-97. doi: 10.1093/glycob/cwv010. Epub 2015 Jan 18.
6
A Photobacterium sp. α2-6-sialyltransferase (Psp2,6ST) mutant with an increased expression level and improved activities in sialylating Tn antigens.一种表达水平提高且在唾液酸化Tn抗原方面活性增强的嗜光菌属α2-6-唾液酸转移酶（Psp2,6ST）突变体。
Carbohydr Res. 2015 May 18;408:127-33. doi: 10.1016/j.carres.2014.12.007. Epub 2014 Dec 23.
7
Synthetic disialyl hexasaccharides protect neonatal rats from necrotizing enterocolitis.合成二唾液酸六糖可保护新生大鼠免受坏死性小肠结肠炎的侵害。
Angew Chem Int Ed Engl. 2014 Jun 23;53(26):6687-91. doi: 10.1002/anie.201403588. Epub 2014 May 21.
8
Can an ancestral condition for milk oligosaccharides be determined? Evidence from the Tasmanian echidna (Tachyglossus aculeatus setosus).能否确定乳寡糖的原始状态？来自塔斯马尼亚针鼹（Tachyglossus aculeatus setosus）的证据。
Glycobiology. 2014 Sep;24(9):826-39. doi: 10.1093/glycob/cwu041. Epub 2014 May 7.
9
One-pot multi-enzyme (OPME) chemoenzymatic synthesis of sialyl-Tn-MUC1 and sialyl-T-MUC1 glycopeptides containing natural or non-natural sialic acid.一锅多酶（OPME）化学酶促合成含有天然或非天然唾液酸的唾液酸化-Tn-MUC1 和唾液酸化-T-MUC1 糖肽。
Bioorg Med Chem. 2013 Aug 15;21(16):4778-85. doi: 10.1016/j.bmc.2013.02.040. Epub 2013 Mar 7.
10
A sialyltransferase mutant with decreased donor hydrolysis and reduced sialidase activities for directly sialylating LewisX.一种唾液酸转移酶突变体，其供体水解活性降低，唾液酸酶活性降低，可直接唾液酸化 LewisX。
ACS Chem Biol. 2012 Jul 20;7(7):1232-40. doi: 10.1021/cb300125k. Epub 2012 May 14.

有效的一锅多酶（OPME）法合成单孔目动物乳寡糖及其他含有4-O-乙酰神经氨酸的唾液酸苷。

Effective one-pot multienzyme (OPME) synthesis of monotreme milk oligosaccharides and other sialosides containing 4-O-acetyl sialic acid.

作者信息

Yu Hai, Zeng Jie, Li Yanhong, Thon Vireak, Shi Baojun, Chen Xi

机构信息

Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.

出版信息

Org Biomol Chem. 2016 Sep 28;14(36):8586-97. doi: 10.1039/c6ob01706a. Epub 2016 Aug 22.

DOI:10.1039/c6ob01706a
PMID:27548611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5036589/
Abstract

A facile one-pot two-enzyme chemoenzymatic approach has been established for the gram (Neu4,5Ac2α3Lac, 1.33 g) and preparative scale (Neu4,5Ac2α3LNnT) synthesis of monotreme milk oligosaccharides. Other O-acetyl-5-N-acetylneuraminic acid (Neu4,5Ac2)- or 4-O-acetyl-5-N-glycolylneuraminic acid (Neu4Ac5Gc) -containing α2-3-sialosides have also been synthesized in the preparative scale. Used as an effective probe, Neu4,5Ac2α3GalβpNP was found to be a suitable substrate by human influenza A viruses but not bacterial sialidases.

摘要

已建立一种简便的一锅双酶化学酶法，用于以克级规模（Neu4,5Ac2α3Lac，1.33克）和制备规模（Neu4,5Ac2α3LNnT）合成单孔目动物乳寡糖。其他含O-乙酰基-5-N-乙酰神经氨酸（Neu4,5Ac2）或4-O-乙酰基-5-N-糖基神经氨酸（Neu4Ac5Gc）的α2-3-唾液酸苷也已在制备规模上合成。作为一种有效的探针，发现Neu4,5Ac2α3GalβpNP是人甲型流感病毒的合适底物，但不是细菌唾液酸酶的底物。