Yu Hai, Zeng Jie, Li Yanhong, Thon Vireak, Shi Baojun, Chen Xi
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
Org Biomol Chem. 2016 Sep 28;14(36):8586-97. doi: 10.1039/c6ob01706a. Epub 2016 Aug 22.
A facile one-pot two-enzyme chemoenzymatic approach has been established for the gram (Neu4,5Ac2α3Lac, 1.33 g) and preparative scale (Neu4,5Ac2α3LNnT) synthesis of monotreme milk oligosaccharides. Other O-acetyl-5-N-acetylneuraminic acid (Neu4,5Ac2)- or 4-O-acetyl-5-N-glycolylneuraminic acid (Neu4Ac5Gc) -containing α2-3-sialosides have also been synthesized in the preparative scale. Used as an effective probe, Neu4,5Ac2α3GalβpNP was found to be a suitable substrate by human influenza A viruses but not bacterial sialidases.
已建立一种简便的一锅双酶化学酶法，用于以克级规模（Neu4,5Ac2α3Lac，1.33克）和制备规模（Neu4,5Ac2α3LNnT）合成单孔目动物乳寡糖。其他含O-乙酰基-5-N-乙酰神经氨酸（Neu4,5Ac2）或4-O-乙酰基-5-N-糖基神经氨酸（Neu4Ac5Gc）的α2-3-唾液酸苷也已在制备规模上合成。作为一种有效的探针，发现Neu4,5Ac2α3GalβpNP是人甲型流感病毒的合适底物，但不是细菌唾液酸酶的底物。
