一锅法三酶合成 UDP-GlcNAc 衍生物。
One-pot three-enzyme synthesis of UDP-GlcNAc derivatives.
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
出版信息
Chem Commun (Camb). 2011 Oct 14;47(38):10815-7. doi: 10.1039/c1cc14034e. Epub 2011 Aug 23.
Abstract
A Pasteurella multocida N-acetylglucosamine 1-phosphate uridylyltransferase (PmGlmU) was cloned and used efficiently with an N-acetylhexosamine 1-kinase (NahK_ATCC55813) and an inorganic pyrophosphatase (PmPpA) for one-pot three-enzyme synthesis of UDP-GlcNAc derivatives with or without further chemical diversification.
摘要
一株多杀性巴氏杆菌 N-乙酰氨基葡萄糖 1-磷酸尿苷酰转移酶（PmGlmU）被克隆，并与 N-乙酰己糖胺 1-激酶（NahK_ATCC55813）和无机焦磷酸酶（PmPpA）一起用于一锅三步酶法合成 UDP-GlcNAc 衍生物，无需进一步的化学衍生化。
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