Suñé C, Jiménez G, Correa I, Bullido M J, Gebauer F, Smerdou C, Enjuanes L
Centro de Biología Molecular, CSIC-UAM Canto Blanco, Madrid, Spain.
Virology. 1990 Aug;177(2):559-69. doi: 10.1016/0042-6822(90)90521-r.
Transmissible gastroenteritis virus (TGEV) was neutralized more than 10(9)-fold with antibodies of a single specificity [monoclonal antibodies (MAbs)]. Most of the virus was neutralized in the first 2-3 min of a reversible reaction, which was followed by a second phase with a decreased neutralization rate and, in some cases, by a persistent fraction, which was a function of the MAb and of the antibody-to-virus ratio. Neutralization of TGEV is a specific event that requires the location of the epitope involved in the neutralization in the appropriate structural context, which is present in the wild-type virus but not in certain MAb escaping mutants. In neutralization of TGEV by binary combinations of MAbs specific for the same or for different antigenic sites, either no cooperation or a synergistic effect, respectively, was observed. Mechanisms of TGEV neutralization by MAbs were characterized at high, intermediate, and low antibody-to-virus ratios. Under these conditions, mainly three steps of the replication cycle were inhibited: binding of virus to the cell, internalization, and a step that takes place after internalization. In addition, virus aggregation could be responsible for the neutralization of 10 to 20% of virus infectivity.
传染性胃肠炎病毒（TGEV）被具有单一特异性的抗体（单克隆抗体[MAbs]）中和超过10⁹倍。在可逆反应的最初2 - 3分钟内，大部分病毒被中和，随后是中和速率降低的第二阶段，在某些情况下，还存在一个持续部分，这是单克隆抗体和抗体与病毒比例的函数。TGEV的中和是一个特定事件，需要参与中和的表位处于适当的结构背景中，这种结构背景存在于野生型病毒中，但不存在于某些逃避单克隆抗体的突变体中。在用针对相同或不同抗原位点的单克隆抗体二元组合中和TGEV时，分别观察到无协同作用或协同效应。在高、中、低抗体与病毒比例下对单克隆抗体中和TGEV的机制进行了表征。在这些条件下，复制周期主要有三个步骤受到抑制：病毒与细胞的结合、内化以及内化后发生的一个步骤。此外，病毒聚集可能导致10%至20%的病毒感染性被中和。
