ASCES College, Caruaruense Association of Higher Education, 55016-400 Caruaru-PE, Brazil.
Molecules. 2011 Jun 7;16(6):4728-39. doi: 10.3390/molecules16064728.
The aims of this study were to quantify the phenolic content and evaluate the antioxidant potential of extracts from the bark and leaves of C. pyramidalis and S. glandulosum. The total phenolic content (TPC) and total tannin content (TTC) were determined using the Folin-Ciocalteu method, and the total flavonoids content (TFC) was measured via complexation with aluminum chloride. The antioxidant activity was evaluated with DPPH (2.2-diphenyl-1-picrylhydrazyl) and FIC (ferrous ion chelating) assays. The TPC ranged between 135.55 ± 9.85 and 459.79 ± 11.65 tannic acid equivalents (TAE) in mg/g material (mg TAE/g). The leaves of both species contained high levels of tannins and flavonoids. The crude ethanol extracts (CEE) from the bark of C. pyramidalis showed high antioxidant activity when compared to ascorbic acid and rutin, whereas the CEE from the leaves was more efficient in chelating ferrous ions. C. pyramidalis had very high phenolic content and anti-radical activity, which indicates a need for further studies aimed at the purification and identification of compounds responsible for the antioxidant activity.
本研究的目的是定量测定肉桂麻和腺体麻的树皮和叶提取物中的酚类含量并评估其抗氧化潜力。采用福林-肖卡法测定总酚含量(TPC)和总单宁含量(TTC),采用与三氯化铝络合的方法测定总类黄酮含量(TFC)。采用 DPPH(2.2-二苯基-1-苦基肼)和 FIC(亚铁离子螯合)测定法评估抗氧化活性。TPC 范围在 135.55±9.85 到 459.79±11.65 没食子酸当量(TAE)/mg 物质(mg TAE/g)。两种植物的叶子都含有高浓度的单宁和类黄酮。与抗坏血酸和芦丁相比,肉桂麻树皮的粗乙醇提取物(CEE)具有较高的抗氧化活性,而腺体麻叶的 CEE 对亚铁离子的螯合作用更强。肉桂麻具有非常高的酚类含量和自由基清除活性,这表明需要进一步研究,以纯化和鉴定具有抗氧化活性的化合物。