• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在体和离体扩散张量成像研究杯状病毒诱导的小鼠胼胝体脱髓鞘

In vivo and ex vivo diffusion tensor imaging of cuprizone-induced demyelination in the mouse corpus callosum.

机构信息

Russel H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

出版信息

Magn Reson Med. 2012 Mar;67(3):750-9. doi: 10.1002/mrm.23032. Epub 2011 Jun 7.

DOI:10.1002/mrm.23032
PMID:21656567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3170659/
Abstract

Diffusion tensor imaging has been widely used in studying rodent models of white matter diseases. In this study, we examined the differences between in vivo and ex vivo fractional anisotropy and diffusivity measurements in the mouse cuprizone model. In the control mouse corpus callosum, ex vivo diffusivities were significantly lower than in vivo measurements, but ex vivo fractional anisotropy values were not significantly different from in vivo fractional anisotropy values. With cuprizone induced demyelination and accompanying pathology in the corpus callosum, changes in in vivo and ex vivo fractional anisotropy and diffusivity measurements were not always in agreement. Our results suggest that ex vivo λ(⟂) was a more reliable indicator of white matter demyelination than in vivo λ(⟂) and in vivo λ(‖) was a more reliable indicator of axonal injury than ex vivo λ(‖) in this model. When comparing in vivo and ex vivo diffusion tensor imaging results of axon and myelin pathology in the rodent models, potential changes in tissue microstructures associated with perfusion fixation should be considered.

摘要

弥散张量成像已广泛应用于研究白质疾病的啮齿动物模型。在本研究中，我们研究了在杯状朊病毒模型中活体和离体各向异性分数和扩散率测量的差异。在对照小鼠胼胝体中，离体扩散率明显低于活体测量值，但离体各向异性分数值与活体各向异性分数值无显著差异。随着胼胝体中脱髓鞘和伴随的病变，活体和离体各向异性分数和扩散率测量的变化并不总是一致的。我们的结果表明，在该模型中，离体 λ(⟂)比活体 λ(⟂)更能可靠地反映白质脱髓鞘，而活体 λ(‖)比离体 λ(‖)更能可靠地反映轴突损伤。在比较活体和离体弥散张量成像结果与啮齿动物模型中的轴突和髓鞘病理时，应考虑与灌注固定相关的组织微结构的潜在变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/77478c4b8385/nihms-295899-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/cf67db7992c2/nihms-295899-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/d089c90dec7e/nihms-295899-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/029d12e3a172/nihms-295899-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/39610e108bdc/nihms-295899-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/6f7bbbf28733/nihms-295899-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/f36d487e4e71/nihms-295899-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/77478c4b8385/nihms-295899-f0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/cf67db7992c2/nihms-295899-f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/d089c90dec7e/nihms-295899-f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/029d12e3a172/nihms-295899-f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/39610e108bdc/nihms-295899-f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/6f7bbbf28733/nihms-295899-f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/f36d487e4e71/nihms-295899-f0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5b03/3170659/77478c4b8385/nihms-295899-f0007.jpg

相似文献

1
In vivo and ex vivo diffusion tensor imaging of cuprizone-induced demyelination in the mouse corpus callosum.在体和离体扩散张量成像研究杯状病毒诱导的小鼠胼胝体脱髓鞘
Magn Reson Med. 2012 Mar;67(3):750-9. doi: 10.1002/mrm.23032. Epub 2011 Jun 7.
2
Quantitative MRI and ultrastructural examination of the cuprizone mouse model of demyelination.脱髓鞘模型中定量 MRI 与超微结构检查。
NMR Biomed. 2013 Nov;26(11):1562-81. doi: 10.1002/nbm.2992. Epub 2013 Aug 13.
3
Histological correlation of diffusional kurtosis and white matter modeling metrics in cuprizone-induced corpus callosum demyelination.铜离子螯合剂诱导胼胝体脱髓鞘中扩散峰度与白质建模指标的组织学相关性
NMR Biomed. 2014 Aug;27(8):948-57. doi: 10.1002/nbm.3140. Epub 2014 Jun 3.
4
Diffusion kurtosis imaging probes cortical alterations and white matter pathology following cuprizone induced demyelination and spontaneous remyelination.扩散峰度成像探究了铜离子螯合剂诱导脱髓鞘和自发髓鞘再生后的皮质改变和白质病变。
Neuroimage. 2016 Jan 15;125:363-377. doi: 10.1016/j.neuroimage.2015.10.052. Epub 2015 Oct 23.
5
Rostrocaudal analysis of corpus callosum demyelination and axon damage across disease stages refines diffusion tensor imaging correlations with pathological features.对胼胝体脱髓鞘和轴突损伤进行头尾分析，可细化扩散张量成像与病理特征的相关性，以反映不同疾病阶段的情况。
J Neuropathol Exp Neurol. 2010 Jul;69(7):704-16. doi: 10.1097/NEN.0b013e3181e3de90.
6
In vivo quantification of demyelination and recovery using compartment-specific diffusion MRI metrics validated by electron microscopy.使用电子显微镜验证的基于体素的弥散 MRI 指标对脱髓鞘和恢复进行体内定量评估。
Neuroimage. 2016 May 15;132:104-114. doi: 10.1016/j.neuroimage.2016.02.004. Epub 2016 Feb 11.
7
Noninvasive detection of cuprizone induced axonal damage and demyelination in the mouse corpus callosum.非侵入性检测小鼠胼胝体中铜螯合剂诱导的轴突损伤和脱髓鞘
Magn Reson Med. 2006 Feb;55(2):302-8. doi: 10.1002/mrm.20774.
8
Demyelination increases radial diffusivity in corpus callosum of mouse brain.脱髓鞘增加了小鼠脑胼胝体的径向扩散率。
Neuroimage. 2005 May 15;26(1):132-40. doi: 10.1016/j.neuroimage.2005.01.028.
9
Quantitative temporal changes in DTI values coupled with histological properties in cuprizone-induced demyelination and remyelination.定量的 DTI 值时间变化与脱髓鞘和髓鞘再生过程中的组织学特性相关。
Neurochem Int. 2018 Oct;119:151-158. doi: 10.1016/j.neuint.2017.10.004. Epub 2017 Oct 10.
10
Quantification of increased cellularity during inflammatory demyelination.炎症性脱髓鞘过程中细胞增生的定量分析。
Brain. 2011 Dec;134(Pt 12):3590-601. doi: 10.1093/brain/awr307.

引用本文的文献

1
Validation of a data-driven multicomponent T2 analysis for quantifying myelin content in the cuprizone mouse model of multiple sclerosis.用于量化多发性硬化症铜剥夺小鼠模型中髓鞘含量的数据驱动多组分T2分析的验证
PLoS One. 2025 May 21;20(5):e0323614. doi: 10.1371/journal.pone.0323614. eCollection 2025.
2
Identification and characterization of the thalamic ventral posterior complex by 11.7T ex vivo diffusion tensor imaging.通过11.7T离体扩散张量成像对丘脑腹后复合体进行识别与特征描述。
Brain Struct Funct. 2025 Apr 15;230(4):49. doi: 10.1007/s00429-025-02915-7.
3
Considerations and recommendations from the ISMRM diffusion study group for preclinical diffusion MRI: Part 2-Ex vivo imaging: Added value and acquisition.

本文引用的文献

1
Three-dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain.三维扩散张量微成像用于小鼠大脑的解剖特征描述。
Magn Reson Med. 2010 Jul;64(1):249-61. doi: 10.1002/mrm.22426.
2
Rostrocaudal analysis of corpus callosum demyelination and axon damage across disease stages refines diffusion tensor imaging correlations with pathological features.对胼胝体脱髓鞘和轴突损伤进行头尾分析，可细化扩散张量成像与病理特征的相关性，以反映不同疾病阶段的情况。
J Neuropathol Exp Neurol. 2010 Jul;69(7):704-16. doi: 10.1097/NEN.0b013e3181e3de90.
3
CXCR2-positive neutrophils are essential for cuprizone-induced demyelination: relevance to multiple sclerosis.
国际磁共振医学学会（ISMRM）扩散研究小组关于临床前扩散磁共振成像的考量与建议：第2部分——离体成像：附加价值与采集
Magn Reson Med. 2025 Jun;93(6):2535-2560. doi: 10.1002/mrm.30435. Epub 2025 Mar 4.
4
Differentiating unirradiated mice from those exposed to conventional or FLASH radiotherapy using MRI.使用磁共振成像（MRI）区分未接受照射的小鼠与接受传统放疗或FLASH放疗的小鼠。
bioRxiv. 2025 Feb 6:2025.02.01.636061. doi: 10.1101/2025.02.01.636061.
5
Intrinsic Gene Expression Correlates of the Biophysically Modeled Diffusion Magnetic Resonance Imaging Signal.生物物理建模扩散磁共振成像信号的内在基因表达相关性
Biol Psychiatry Glob Open Sci. 2024 Dec 5;5(2):100430. doi: 10.1016/j.bpsgos.2024.100430. eCollection 2025 Mar.
6
Quantitative Analysis of Early White Matter Damage in Cuprizone Mouse Model of Demyelination Using 7.0 T MRI Multiparametric Approach.采用 7.0T MRI 多参数方法对脱髓鞘杯状蛋白模型小鼠早期白质损伤的定量分析。
ASN Neuro. 2024;16(1):2404366. doi: 10.1080/17590914.2024.2404366. Epub 2024 Oct 14.
7
Magnetic resonance imaging of white matter response to diesel exhaust particles.白质对柴油尾气颗粒反应的磁共振成像
Res Sq. 2023 Jul 18:rs.3.rs-3087503. doi: 10.21203/rs.3.rs-3087503/v1.
8
Effects of anesthesia exposure on postnatal maturation of white matter in rhesus monkeys.麻醉暴露对恒河猴出生后白质成熟的影响。
Dev Psychobiol. 2023 Jul;65(5):e22396. doi: 10.1002/dev.22396.
9
Evaluation of kernel low-rank compressed sensing in preclinical diffusion magnetic resonance imaging.临床前扩散磁共振成像中内核低秩压缩感知的评估
Front Neurosci. 2023 Jun 2;17:1172830. doi: 10.3389/fnins.2023.1172830. eCollection 2023.
10
Utilization of potato starch suspension for MR-microimaging in mouse embryos.马铃薯淀粉悬浮液在小鼠胚胎磁共振显微成像中的应用。
iScience. 2022 Nov 30;25(12):105694. doi: 10.1016/j.isci.2022.105694. eCollection 2022 Dec 22.
CXCR2 阳性中性粒细胞对于 cuprizone 诱导的脱髓鞘至关重要：与多发性硬化症相关。
Nat Neurosci. 2010 Mar;13(3):319-26. doi: 10.1038/nn.2491. Epub 2010 Feb 14.
4
The cuprizone animal model: new insights into an old story.铜灰模型动物：旧故事中的新见解。
Acta Neuropathol. 2009 Dec;118(6):723-36. doi: 10.1007/s00401-009-0591-3. Epub 2009 Sep 18.
5
Aldehyde fixative solutions alter the water relaxation and diffusion properties of nervous tissue.醛类固定剂溶液会改变神经组织的水弛豫和扩散特性。
Magn Reson Med. 2009 Jul;62(1):26-34. doi: 10.1002/mrm.21977.
6
Diffusion tensor magnetic resonance imaging of Wallerian degeneration in rat spinal cord after dorsal root axotomy.背根轴突切断术后大鼠脊髓华勒氏变性的扩散张量磁共振成像
J Neurosci. 2009 Mar 11;29(10):3160-71. doi: 10.1523/JNEUROSCI.3941-08.2009.
7
Axial diffusivity is the primary correlate of axonal injury in the experimental autoimmune encephalomyelitis spinal cord: a quantitative pixelwise analysis.轴向扩散率是实验性自身免疫性脑脊髓炎脊髓轴突损伤的主要相关因素：一项定量逐像素分析。
J Neurosci. 2009 Mar 4;29(9):2805-13. doi: 10.1523/JNEUROSCI.4605-08.2009.
8
Recovery from chronic demyelination by thyroid hormone therapy: myelinogenesis induction and assessment by diffusion tensor magnetic resonance imaging.甲状腺激素疗法促进慢性脱髓鞘恢复：髓鞘形成诱导及扩散张量磁共振成像评估
J Neurosci. 2008 Dec 24;28(52):14189-201. doi: 10.1523/JNEUROSCI.4453-08.2008.
9
Postmortem interval alters the water relaxation and diffusion properties of rat nervous tissue--implications for MRI studies of human autopsy samples.死后间隔时间会改变大鼠神经组织的水弛豫和扩散特性——对人类尸检样本的MRI研究的启示。
Neuroimage. 2009 Feb 1;44(3):820-6. doi: 10.1016/j.neuroimage.2008.09.054. Epub 2008 Oct 19.
10
Monitoring demyelination and remyelination by magnetization transfer imaging in the mouse brain at 9.4 T.在9.4 T磁场下通过磁共振成像监测小鼠脑内的脱髓鞘和髓鞘再生。
MAGMA. 2008 Sep;21(5):357-62. doi: 10.1007/s10334-008-0141-3. Epub 2008 Sep 9.