Barraclough R, Fernig D G, Rudland P S, Smith J A
Department of Biochemistry, University of Liverpool, England.
J Cell Physiol. 1990 Aug;144(2):333-44. doi: 10.1002/jcp.1041440220.
Acidic fibroblast growth factor (aFGF) mRNA was detected in a rat mammary fibroblastic cell line, but not in rat mammary epithelial cell lines or myoepithelial-like cell lines. Basic FGF (bFGF) mRNA was detected in both the fibroblasts and the myoepithelial-like cells, but was absent from the epithelial cells. A series of cell lines representing stages in the differentiation pathway of epithelial cells to a myoepithelial-like morphology showed an increase in the amount of bFGF mRNA and activity present and the FGF from the myoepithelial-like rat mammary 29 cells was able to displace [125I]-bFGF specifically bound to rat mammary fibroblasts. FGF activity was also present in an extract of rat mammary gland. Analysis of cell extracts and conditioned medium indicated that FGF activity was cell-associated. The cell-associated bFGF was resistant to degradation by trypsin. Extraction of myoepithelial-like cells with Triton X-100 and 2 M NaCl showed that 50-65% of the cell-associated bFGF was in a detergent-resistant but 2 M NaCl-labile structure. Thus, the synthesis of bFGF is developmentally regulated in rat mammary cell lines, and at least 50% is present in the extracellular matrix.
在大鼠乳腺成纤维细胞系中检测到酸性成纤维细胞生长因子（aFGF）mRNA，但在大鼠乳腺上皮细胞系或肌上皮样细胞系中未检测到。碱性成纤维细胞生长因子（bFGF）mRNA在成纤维细胞和肌上皮样细胞中均被检测到，但上皮细胞中不存在。一系列代表上皮细胞向肌上皮样形态分化途径各阶段的细胞系显示，bFGF mRNA的量和活性增加，并且来自大鼠乳腺肌上皮样29细胞的FGF能够特异性取代与大鼠乳腺成纤维细胞特异性结合的[125I]-bFGF。FGF活性也存在于大鼠乳腺提取物中。对细胞提取物和条件培养基的分析表明，FGF活性与细胞相关。细胞相关的bFGF对胰蛋白酶降解具有抗性。用Triton X-100和2M NaCl提取肌上皮样细胞表明，50-65%的细胞相关bFGF处于抗去污剂但对2M NaCl不稳定的结构中。因此，bFGF的合成在大鼠乳腺细胞系中受到发育调控，并且至少50%存在于细胞外基质中。
