采用荧光灶减少试验检测儿童粪便中轮状病毒中和抗体
Measurement of rotavirus-neutralizing coproantibody in children by fluorescent focus reduction assay.
作者信息
Coulson B S, Masendycz P J
机构信息
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
出版信息
J Clin Microbiol. 1990 Jul;28(7):1652-4. doi: 10.1128/jcm.28.7.1652-1654.1990.
Abstract
A fluorescent focus reduction assay suitable for the measurement of rotavirus-neutralizing antibodies in the feces of children was developed. Of 408 stools tested, 7% showed false-positive neutralization, and the number of rotavirus serotypes neutralized by a fecal extract was proportional to the levels of antirotaviral immunoglobulin A in the extract.
摘要
开发了一种适用于检测儿童粪便中轮状病毒中和抗体的荧光灶减少试验。在检测的408份粪便样本中，7%显示假阳性中和，粪便提取物中和的轮状病毒血清型数量与提取物中抗轮状病毒免疫球蛋白A的水平成正比。
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