Brüssow H, Offit P A, Sidoti J
Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, Lausanne, Switzerland.
J Clin Microbiol. 1991 May;29(5):869-73. doi: 10.1128/jcm.29.5.869-873.1991.
Serum samples from 870 Ecuadorian children who underwent natural rotavirus exposure were tested for neutralizing serum antibody to heterologous animal rotavirus (RV) serotypes. Six percent of the sera neutralized porcine RV OSU (serotype 5), 10% neutralized bovine RV NCDV (serotype 6), 4% neutralized avian RV Ch-2 (serotype 7), and 8% neutralized bovine RV V1005 (serotype 10). Neutralization was defined as a 90% reduction in infectious virus at a 1:100 serum dilution. The prevalence of antibody to all four heterotypic viruses increased with the age of the children and the number of human RV serotypes neutralized, but prevalences did not differ significantly between children from rural and urban areas of Ecuador. No serum sample that specifically neutralized bovine RV NCDV was identified. We inferred from the seroepidemiological analysis that human RVs contain immunorecessive neutralization epitopes that can stimulate cross-neutralizing antibody to heterotypic animal RVs. This occurs increasingly with age and with the number of human serotypes recognized by a child's neutralizing antibody. Thus, it appears that a broadened immune response to the heterotypic strains occurs with repetitive RV infections.
对870名自然感染轮状病毒的厄瓜多尔儿童的血清样本进行检测，以确定其对异源动物轮状病毒（RV）血清型的中和血清抗体。6%的血清可中和猪RV OSU（血清型5），10%可中和牛RV NCDV（血清型6），4%可中和禽RV Ch-2（血清型7），8%可中和牛RV V1005（血清型10）。中和定义为在1:100血清稀释度下感染性病毒减少90%。对所有四种异型病毒的抗体流行率随儿童年龄和中和的人RV血清型数量增加而升高，但厄瓜多尔农村和城市地区儿童的流行率无显著差异。未鉴定出可特异性中和牛RV NCDV的血清样本。我们从血清流行病学分析推断，人RV含有免疫隐性中和表位，可刺激产生针对异型动物RV的交叉中和抗体。这种情况随年龄增长以及儿童中和抗体识别的人血清型数量增加而越来越多地发生。因此，似乎反复感染RV会导致对异型毒株产生更广泛的免疫反应。
