Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn T A, Sakmann B, Seeburg P H
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, F.R.G.
Science. 1990 Aug 3;249(4968):556-60. doi: 10.1126/science.2166337.
Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library. In situ hybridization revealed differential expression patterns of the cognate mRNAs throughout the brain. Functional expression of the cDNAs in cultured mammalian cells generated receptors displaying alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-selective binding pharmacology (AMPA = quisqualate greater than glutamate greater than kainate) as well as cation channels gated by glutamate, AMPA, and kainate and blocked by 6,7-dinitroquinoxaline-2,3-dione (CNQX).
从大鼠脑cDNA文库中分离出四个编码900个氨基酸的假定谷氨酸受体的克隆cDNA，其序列同一性约为70%。原位杂交显示同源mRNA在整个大脑中的表达模式存在差异。这些cDNA在培养的哺乳动物细胞中的功能表达产生了具有α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）选择性结合药理学特性（AMPA = 喹啉酸大于谷氨酸大于海人藻酸）的受体，以及由谷氨酸、AMPA和海人藻酸门控并被6,7-二硝基喹喔啉-2,3-二酮（CNQX）阻断的阳离子通道。
