Manning P J, Rokach J, Malo J L, Ethier D, Cartier A, Girard Y, Charleson S, O'Byrne P M
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
J Allergy Clin Immunol. 1990 Aug;86(2):211-20. doi: 10.1016/s0091-6749(05)80068-5.
The sulphidopeptide leukotrienes C4 and D4 (LTC4, LTD4) are potent bronchoconstrictor mediators, released from human lung fragments after challenge with specific allergens in vitro. The purpose of this study was to measure urinary LTE4 (metabolite of LTC4 and LTD4) in subjects undergoing inhalation challenges with allergens or occupational sensitizing agents in the laboratory. Eighteen subjects with previously documented isolated early asthmatic responses (EARs), isolated late asthmatic responses (LARs), or dual (both early and late) asthmatic responses were studied. Urinary LTE4 levels increased in subjects who developed either isolated EARs (mean fall in FEV1, 27.98%) or early responses preceding LARs (mean fall in FEV1, 15.01%). The baseline levels of LTE4 were 150.26 (SEM, 49.5) pg/mg of creatinine in the isolated responders and 66.60 (SEM, 13.5) pg/mg of creatinine in the dual responders. These levels increased to 1816 (SEM, 606.1) pg/mg of creatinine (p = 0.041) and 174.80 (SEM, 40.1) pg/mg of creatinine (p = 0.025), respectively, after the EAR. The degree of maximal bronchoconstriction during the EAR correlated with the levels of LTE4 (r = 0.68; p = 0.001). No significant increase in urinary LTE4 levels occurred during the LAR. These results suggest that the LTE4 precursors, LTC4 and LTD4, are important bronchoconstrictor mediators causing EARs after allergen inhalation.
硫化肽白三烯C4和D4（LTC4、LTD4）是强效支气管收缩介质，在体外受到特定过敏原刺激后，可从人肺组织碎片中释放出来。本研究的目的是测定在实验室中接受过敏原或职业致敏剂吸入激发试验的受试者尿液中的LTE4（LTC4和LTD4的代谢产物）水平。对18名先前记录有孤立性早期哮喘反应（EAR）、孤立性晚期哮喘反应（LAR）或双重（早期和晚期）哮喘反应的受试者进行了研究。出现孤立性EAR（FEV1平均下降27.98%）或LAR之前的早期反应（FEV1平均下降15.01%）的受试者尿液LTE4水平升高。孤立性反应者的LTE4基线水平为150.26（标准误，49.5）pg/mg肌酐，双重反应者为66.60（标准误，13.5）pg/mg肌酐。EAR后，这些水平分别升至1816（标准误，606.1）pg/mg肌酐（p = 0.041）和174.80（标准误，40.1）pg/mg肌酐（p = 0.025）。EAR期间最大支气管收缩程度与LTE4水平相关（r = 0.68；p = 0.001）。LAR期间尿液LTE4水平无显著升高。这些结果表明，LTE4的前体LTC4和LTD4是过敏原吸入后引起EAR的重要支气管收缩介质。
