在人脑脊液中检测到几种新型含γ-氨基丁酸的化合物。

Detection of several novel gamma-aminobutyric acid-containing compounds in human CSF.

作者信息

Miller J M, Ferraro T N, Hare T A

机构信息

Department of Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.

出版信息

J Neurochem. 1990 Sep;55(3):769-73. doi: 10.1111/j.1471-4159.1990.tb04558.x.

DOI:10.1111/j.1471-4159.1990.tb04558.x

PMID:2166773

Abstract

gamma-Aminobutyric acid (GABA) concentrations in human CSF are known to increase significantly after hydrolysis; however, the source of this increase has been unknown. Using either ion-exchange or reverse-phase chromatography coupled with on-line alkaline hydrolysis, we have shown 2-pyrrolidinone, the lactam of GABA, to be present in insufficient quantity to account for this increase. Subsequent experiments involving fraction collection of column eluents followed by acid hydrolysis and rechromatography demonstrated the presence of several previously undetected GABA-containing compounds.

摘要

已知人脑脊液中的γ-氨基丁酸（GABA）浓度在水解后会显著增加；然而，这种增加的来源尚不清楚。使用离子交换或反相色谱结合在线碱性水解，我们发现GABA的内酰胺2-吡咯烷酮的含量不足以解释这种增加。随后的实验包括收集柱洗脱液的馏分，然后进行酸水解和重新色谱分析，结果表明存在几种以前未检测到的含GABA的化合物。