Institut für Biowissenschaften, Pflanzengenetik, Universität Rostock, Albert-Einstein-Strasse 3, Rostock, Germany.
Plant Biol (Stuttg). 2011 Jul;13(4):632-42. doi: 10.1111/j.1438-8677.2010.00426.x. Epub 2011 Feb 5.
An easy and manageable in vitro screening system for drought tolerance of sunflower seedlings based on MS media supplemented with polyethylene glycol 6000 was evaluated. Morphological and physiological parameters were compared between control (-0.05 MPa) and drought-stressed (-0.6 MPa) seedlings of Helianthus annuus L. cv. Peredovick. There was a significant growth deficit in drought-stressed plants compared to control plants in terms of hypocotyl length, and shoot and root fresh mass. Shoot growth was more restricted than root growth, resulting in an increased root/shoot ratio of drought-stressed plants. Accumulation of osmolytes such as inositol (65-fold), glucose (58-fold), proline (55-fold), fructose (11-fold) and sucrose (eightfold), in leaves of drought-stressed plants could be demonstrated by gas-liquid chromatography. Soluble protein patterns of leaves were analysed with two-dimensional gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry. A set of 46 protein spots allowed identification of 19 marker proteins. Quantitative changes in protein expression of drought-stressed versus control plants were detected. In leaves of drought-stressed sunflower seedlings six proteins were significantly up-regulated more than twofold: a putative caffeoyl-CoA 3-O-methyltransferase (4.5-fold), a fructokinase 3 (3.3-fold), a vegetative storage protein (2.5-fold), a glycine-rich RNA binding protein (2.2-fold), a CuZn-superoxide dismutase (2.1-fold) and an unknown low molecular weight protein (2.3-fold). These proteins represent general stress proteins induced under drought conditions or proteins contributing to basic carbon metabolism. The up-regulated proteins are interesting candidates for further physiological and molecular investigations regarding drought tolerance in sunflower.
评估了一种基于添加聚乙二醇 6000 的 MS 培养基的向日葵幼苗耐旱性的简便可控体外筛选系统。对 Helianthus annuus L. cv. Peredovick 的对照（-0.05 MPa）和干旱胁迫（-0.6 MPa）幼苗的形态和生理参数进行了比较。与对照植株相比，干旱胁迫植株的下胚轴长度、地上部和根鲜重均显著减少。与根生长相比，地上部生长受到更大的限制，导致干旱胁迫植株的根/地上部比例增加。通过气相色谱法可以证明干旱胁迫植株叶片中肌醇（65 倍）、葡萄糖（58 倍）、脯氨酸（55 倍）、果糖（11 倍）和蔗糖（8 倍）等渗透物的积累。用二维凝胶电泳（2D-PAGE）和 MALDI-TOF 质谱分析叶片的可溶性蛋白图谱。一组 46 个蛋白斑点允许鉴定 19 个标记蛋白。检测到干旱胁迫与对照植株之间的蛋白质表达定量变化。在干旱胁迫的向日葵幼苗叶片中，有 6 种蛋白质的表达显著上调了两倍以上：一种假定的咖啡酰辅酶 A 3-O-甲基转移酶（4.5 倍）、一种果糖激酶 3（3.3 倍）、一种营养贮藏蛋白（2.5 倍）、一种甘氨酸丰富的 RNA 结合蛋白（2.2 倍）、一种 CuZn-超氧化物歧化酶（2.1 倍）和一种未知的低分子量蛋白（2.3 倍）。这些蛋白质代表在干旱条件下诱导的一般应激蛋白或对基础碳代谢有贡献的蛋白质。上调的蛋白质是进一步研究向日葵耐旱性的生理和分子的有趣候选者。
