环状芽孢杆菌β-半乳糖苷酶基因的克隆与表达
Cloning and expression of a β-galactosidase gene of Bacillus circulans.
作者信息
Song Jingyuan, Imanaka Hiroyuki, Imamura Koreyoshi, Minoda Masashi, Katase Toru, Hoshi Yukiko, Yamaguchi Shotaro, Nakanishi Kazuhiro
机构信息
Graduate School of Natural Science and Technology, Okayama University, Kita-ku, Okayama, Japan.
出版信息
Biosci Biotechnol Biochem. 2011;75(6):1194-7. doi: 10.1271/bbb.110014. Epub 2011 Jun 13.
A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta(®). Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
基于从商业酶制剂Biolacta(®)中分离得到的N端和内部肽段序列，对环状芽孢杆菌ATCC 31382的β-半乳糖苷酶基因进行了克隆和测序。利用克隆得到的基因，重组β-半乳糖苷酶及其缺失突变体在大肠杆菌细胞中作为His标签蛋白进行了过量表达，并对表达的酶进行了表征。
Zotero 插件