Huang Huey-Chun, Chiu Shao-Hua, Chang Tsong-Min
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan.
Biosci Biotechnol Biochem. 2011;75(6):1067-72. doi: 10.1271/bbb.100851. Epub 2011 Jun 13.
[6]-Gingerol is an active component of ginger that shows antipyretic and anti-inflammation activities. To find a novel skin-whitening agent, the melanogeneis inhibitory effects and action mechanisms of [6]-gingerol were investigated. In the present study, the effects of [6]-gingerol on mushroom tyrosinase, tyrosinase activity, and melanin content were determined spectrophotometrically, and the expression of tyrosinase and related proteins in B16F10 murine melanoma cells was evaluated by Western blotting. Furthermore, a possible signaling pathway involved in [6]-gingerolmediated depigmentation was investigated by means of specific inhibitors. The results revealed that [6]-gingerol (25-100 µM) effectively suppresses murine tyrosinase activity and decreases the amount of melanin in a dose-dependent manner. Additionally, it also effectively decreased the intracellular reactive oxygen species (ROS) level in a dose-dependent pattern in the same dose range. Our results indicate that [6]-gingerol inhibits melanogenesis of B16F10 melanoma and can function as a good skinwhitening agent.
[6]-姜辣素是生姜的一种活性成分，具有解热和抗炎活性。为了寻找一种新型的皮肤美白剂，研究了[6]-姜辣素对黑色素生成的抑制作用及其作用机制。在本研究中，采用分光光度法测定了[6]-姜辣素对蘑菇酪氨酸酶、酪氨酸酶活性和黑色素含量的影响，并通过蛋白质免疫印迹法评估了[6]-姜辣素对B16F10小鼠黑色素瘤细胞中酪氨酸酶及相关蛋白表达的影响。此外，利用特异性抑制剂研究了[6]-姜辣素介导的色素沉着可能涉及的信号通路。结果表明，[6]-姜辣素（25-100μM）能有效抑制小鼠酪氨酸酶活性，并以剂量依赖的方式减少黑色素的含量。此外，在相同剂量范围内，它还能以剂量依赖的方式有效降低细胞内活性氧（ROS）水平。我们的结果表明，[6]-姜辣素可抑制B16F10黑色素瘤细胞的黑色素生成，有望成为一种优良的皮肤美白剂。
