Department of Food Science and Technology, State University of Londrina, Curitiba-Paraná, Brazil.
J Appl Microbiol. 2011 Sep;111(3):739-48. doi: 10.1111/j.1365-2672.2011.05074.x. Epub 2011 Jul 12.
Considering the agronomic and industrial damage that is caused by the fungus Fusarium graminearum, as well as the serious health risks it poses to humans and animals exposed to F. graminearum-produced mycotoxin deoxynivalenol (DON), this study evaluated the ability of different lactic acid bacteria (LAB) strains to inhibit fungal development and remove DON in vitro.
The antagonistic effects of strains and commercial cultures of LAB were evaluated against F. graminearum IAPAR 2218 by the agar diffusion method. Additionally, the influence of the culture media, pH and the presence of lactic and acetic acid on these effects was tested. The capacity to remove DON by viable cells and heat-inactivated cells was analysed in liquid media and quantified by high performance liquid chromatography (HPLC). All isolated strains and commercial cultures inhibited the fungus and removed DON. The pH and culture media concentration did not influence these abilities, but heat inactivation had a strong effect on the ability of bacteria to remove mycotoxin.
The isolated bacteria are able to inhibit F. graminearum growth and remove DON in vitro.
This study suggests potential application of the isolated LAB strains in the inhibition of F. graminearum IAPAR 2218 and DON removal in vitro.
考虑到真菌禾谷镰刀菌造成的农艺和工业损害，以及人类和动物接触到由禾谷镰刀菌产生的真菌毒素脱氧雪腐镰刀菌烯醇（DON）而带来的严重健康风险，本研究评估了不同乳酸菌（LAB）菌株在体外抑制真菌生长和去除 DON 的能力。
通过琼脂扩散法评估了菌株和商业培养的 LAB 对禾谷镰刀菌 IAPAR 2218 的拮抗作用。此外，还测试了培养基、pH 值以及乳酸和乙酸的存在对这些作用的影响。在液体培养基中分析了活菌和热灭活细胞去除 DON 的能力，并通过高效液相色谱法（HPLC）进行定量分析。所有分离的菌株和商业培养物均抑制了真菌并去除了 DON。pH 值和培养基浓度对这些能力没有影响，但热失活对细菌去除真菌毒素的能力有很大影响。
分离的细菌能够抑制禾谷镰刀菌 IAPAR 2218 的生长并在体外去除 DON。
本研究表明，分离的 LAB 菌株可能在体外抑制禾谷镰刀菌 IAPAR 2218 和去除 DON 方面具有潜在的应用价值。
