Pestka J J, el-Bahrawy A, Hart L P
Mycopathologia. 1985 Jul;91(1):23-8. doi: 10.1007/BF00437282.
Growth and toxigenesis by Fusarium graminearum R6576, were compared in four liquid media. Parameters monitored during the fermentation were deoxynivalenol (DON) and 15-acetyl deoxynivalenol (15-ADON) production, fungal mass, carbohydrate utilization, and pH. Factors which were varied included basal medium composition, corn steep liquor (CSL) concentration, sucrose concentration and ammonium tartrate concentration. Growth in modified Fries medium resulted in only low levels of DON (0.25 mg/L) and 15-ADON (0.25 mg/L) after 20 days. Addition of 4% CSL to modified Fries medium raised the 20 day DON yield to 16.5 mg/l. Increasing the sucrose concentration in modified Fries medium amended with 4% CSL resulted in increased mycelial dry weight but decreased levels of DON. Concentrations of ammonium tartrate greater than 1% in modified Fries amended with 4% CSL greatly reduced DON yield. Use of glucose-yeast extract-peptone (GYEP) for toxin production resulted in higher yields of 15-ADON (14.0 mg/L) than DON (5.5 mg/L) after 20 days. However, supplementation of GYEP with 4% CSL resulted primarily in DON production (4.5 mg/L) after 20 days. In general, qualitative and quantitative production of DON and 15-ADON by Fusarium graminearum R6576 were dependent on the composition of the complex liquid medium.
在四种液体培养基中对禾谷镰刀菌R6576的生长和产毒情况进行了比较。发酵过程中监测的参数包括脱氧雪腐镰刀菌烯醇（DON）和15 - 乙酰脱氧雪腐镰刀菌烯醇（15 - ADON）的产生、真菌量、碳水化合物利用情况以及pH值。变化的因素包括基础培养基组成、玉米浆（CSL）浓度、蔗糖浓度和酒石酸铵浓度。在改良弗里斯培养基中培养20天后，DON（0.25毫克/升）和15 - ADON（0.25毫克/升）的产量仅处于较低水平。向改良弗里斯培养基中添加4%的CSL可使20天的DON产量提高至16.5毫克/升。在添加了4% CSL的改良弗里斯培养基中增加蔗糖浓度，会导致菌丝体干重增加，但DON水平降低。在添加了4% CSL的改良弗里斯培养基中，酒石酸铵浓度大于1%会大幅降低DON产量。使用葡萄糖 - 酵母提取物 - 蛋白胨（GYEP）产毒，20天后15 - ADON（14.0毫克/升）的产量高于DON（5.5毫克/升）。然而，在GYEP中添加4%的CSL，20天后主要产生DON（4.5毫克/升）。总体而言，禾谷镰刀菌R6576产生DON和15 - ADON的定性和定量情况取决于复合液体培养基的组成。
