Bale J F, O'Neil M E, Lyon B, Perlman S
Department of Pediatrics, University of Iowa College of Medicine, Iowa City.
Invest Ophthalmol Vis Sci. 1990 Aug;31(8):1575-81.
To investigate the pathogenesis of ocular cytomegalovirus infections, 3-week-old BALB/c mice were inoculated with 10(4) plaque-forming units of murine cytomegalovirus (MCMV) by the right anterior chamber and studied sequentially with the use of virus assays and in situ nucleic acid hybridization methods. During acute infection, MCMV was recovered from the right vitreous, lens, cornea, retina/choroid, and optic nerve. Titers of MCMV exceeded 10(3) per ml of homogenate on days 4 and 7 after inoculation. With the use of biotinylated MCMV DNA probes, MCMV nucleic acids were detected in and adjacent to cells of the iris and ciliary body and occasionally within inflammatory lesions of the cornea. During chronic infection, MCMV was recovered, with the use of co-cultivation or explant methods, from ocular tissues of occasional mice inoculated with MCMV 1 yr earlier. Infectious MCMV was also recovered from the ocular homogenates of a group of mice immunosuppressed with antilymphocyte serum and cortisone. These studies indicate that cells of the uveal tract are permissive for MCMV and suggest that intrinsic persistence or latency of cytomegalovirus in ocular tissues could contribute to the pathogenesis of ocular infections in immunosuppressed hosts.
为了研究眼部巨细胞病毒感染的发病机制，将10⁴个鼠巨细胞病毒（MCMV）蚀斑形成单位接种到3周龄的BALB/c小鼠右前房，并依次使用病毒检测和原位核酸杂交方法进行研究。在急性感染期间，从右玻璃体、晶状体、角膜、视网膜/脉络膜和视神经中分离出MCMV。接种后第4天和第7天，MCMV滴度超过每毫升匀浆10³。使用生物素化的MCMV DNA探针，在虹膜和睫状体细胞及其附近以及偶尔在角膜的炎性病变中检测到MCMV核酸。在慢性感染期间，通过共培养或外植体方法，从1年前接种MCMV的部分小鼠眼部组织中分离出MCMV。还从一组用抗淋巴细胞血清和可的松免疫抑制的小鼠眼部匀浆中分离出有传染性的MCMV。这些研究表明，葡萄膜细胞对MCMV易感，并提示巨细胞病毒在眼部组织中的内在持续存在或潜伏可能导致免疫抑制宿主眼部感染的发病机制。
