Palma Matteo, Abramson Justin J, Gorodetsky Alon A, Nuckolls Colin, Sheetz Michael P, Wind Shalom J, Hone James
Department of Mechanical Engineering & Applied Physics and Applied Mathematics, Columbia University, New York, NY, USA.
Methods Mol Biol. 2011;749:169-85. doi: 10.1007/978-1-61779-142-0_12.
Nanopatterned arrays of biomolecules are a powerful tool to address fundamental issues in many areas of biology. DNA nanoarrays, in particular, are of interest in the study of DNA-protein interactions and for biodiagnostic investigations. In this context, achieving a highly specific nanoscale assembly of oligonucleotides at surfaces is critical. In this chapter, we describe a method to control the immobilization of DNA on nanopatterned surfaces; the nanofabrication and the bio-functionalization involved in the process will be discussed.
生物分子的纳米图案阵列是解决生物学许多领域基本问题的有力工具。特别是DNA纳米阵列，在DNA-蛋白质相互作用研究和生物诊断研究中备受关注。在这种情况下，在表面实现寡核苷酸的高度特异性纳米级组装至关重要。在本章中，我们描述了一种控制DNA在纳米图案表面固定的方法；将讨论该过程中涉及的纳米制造和生物功能化。
