Institute of Organic Chemistry and Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria.
Anal Chem. 2011 Jul 15;83(14):5754-61. doi: 10.1021/ac201123t. Epub 2011 Jun 28.
The ambient mass spectrometry technique, desorption electrospray ionization mass spectrometry (DESI-MS), is applied for the rapid identification and spatially resolved relative quantification of chlorophyll degradation products in complex senescent plant tissue matrixes. Polyfunctionalized nonfluorescent chlorophyll catabolites (NCCs), the "final" products of the chlorophyll degradation pathway, are detected directly from leaf tissues within seconds and structurally characterized by tandem mass spectrometry (MS/MS) and reactive-DESI experiments performed in situ. The sensitivity of DESI-MS analysis of these compounds from degreening leaves is enhanced by the introduction of an imprinting technique. Porous polytetrafluoroethylene (PTFE) is used as a substrate for imprinting the leaves, resulting in increased signal intensities compared with those obtained from direct leaf tissue analysis. This imprinting technique is used further to perform two-dimensional (2D) imaging mass spectrometry by DESI, producing well-resolved images of the spatial distribution of NCCs in senescent leaf tissues.
环境质谱技术，解吸电喷雾电离质谱（DESI-MS），被应用于快速鉴定和空间分辨复杂衰老植物组织基质中叶绿素降解产物的相对定量。多功能非荧光叶绿素分解产物（NCCs）是叶绿素降解途径的“最终”产物，可在几秒钟内直接从叶片组织中检测到，并通过串联质谱（MS/MS）和原位反应性 DESI 实验进行结构表征。通过引入印迹技术，增强了从脱绿叶片中这些化合物的 DESI-MS 分析的灵敏度。多孔聚四氟乙烯（PTFE）用作印迹叶片的基质，与直接从叶片组织分析获得的信号强度相比，其信号强度得到增强。该印迹技术进一步用于通过 DESI 进行二维（2D）成像质谱，在衰老叶片组织中产生 NCC 空间分布的高分辨率图像。
