Mrowicka Malgorzata, Mrowicki Jerzy, Mik Michal, Wojtczak Radoslaw, Dziki Lukasz, Dziki Adam, Majsterek Ireneusz
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, Hallera 1 Square, Lodz 90-647, Poland.
Chair of Surgery, Department of General and Colorectal Surgery, Medical University of Lodz, Hallera 1 Square, Lodz 90-647, Poland.
Oncotarget. 2017 Nov 27;8(65):109332-109339. doi: 10.18632/oncotarget.22675. eCollection 2017 Dec 12.
The main aim of this study was investigate the association between the genetic polymorphism of antioxidant enzyme genes: and and the risk of inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis in the Polish population.
A total of 445 subjects including 200 patients with IBD and 245 controls were allowed in this study. We determined activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx1) and examination their association with the SNPs of respective genes ( +35A/C, C-262T and Pro197Leu). RFLP technique was used to determine the selected genes polymorphisms. Antioxidant enzymes activity were evaluated in erythrocyte hemolysate of 23 patients with non-active IBD and 30 healthy participants.
The A/C genotype and the C allele frequencies of A/C polymorphism of gene were significantly associated with the reduced risk of IBD (OR=0.43; 95% CI 0.23; 0.83). Alike, C/T (OR=0.45; 95% CI= 0.29; 0.70) and T/T genotype (OR=0.43; 95% CI= 0.21; 0.87) of gene polymorphism diminished the susceptibility to IBD. A significant decrease of CAT (=0.028) and increase of GPx1 (=0.025) enzyme activities were seen in IBD patients compared to control.
Our data confirm dysregulated antioxidant capacity in patients suffering from IBD. Both, the A/C genotype as well as C/T and T/T genotypes may be associated with a reduction risk of IBD in the Polish population.
本研究的主要目的是调查抗氧化酶基因的基因多态性与炎症性肠病（IBD）风险之间的关联，IBD包括波兰人群中的克罗恩病和溃疡性结肠炎。
本研究共纳入445名受试者，其中包括200例IBD患者和245名对照。我们测定了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx1）的活性，并检测了它们与各自基因单核苷酸多态性（+35A/C、C-262T和Pro197Leu）的关联。采用限制性片段长度多态性（RFLP）技术确定所选基因的多态性。在23例非活动期IBD患者和30名健康参与者的红细胞溶血产物中评估抗氧化酶活性。
基因A/C多态性的A/C基因型和C等位基因频率与IBD风险降低显著相关（OR=0.43；95%可信区间0.23；0.83）。同样，基因多态性的C/T（OR=0.45；95%可信区间=0.29；0.70）和T/T基因型（OR=0.43；95%可信区间=0.21；0.87）降低了IBD易感性。与对照组相比，IBD患者中CAT（=0.028）酶活性显著降低，GPx1（=0.025）酶活性升高。
我们的数据证实IBD患者抗氧化能力失调。基因的A/C基因型以及基因的C/T和T/T基因型可能与波兰人群中IBD风险降低有关。
