Neurobiology and Pain Therapeutics Section, Laboratory of Sensory Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
Expert Rev Proteomics. 2011 Jun;8(3):309-16. doi: 10.1586/epr.11.23.
B-cell-mediated humoral responses are triggered in many human diseases, including autoimmune diseases, cancer, and neurologic and infectious diseases. However, the full exploitation of the information contained within a patient's antibody repertoire for diagnosis, monitoring and even disease prediction has been limited due to the poor diagnostic performance of many immunoassay formats. We have developed luciferase immunoprecipitation systems (LIPS) that harnesses light-emitting proteins to generate high-definition antibody profiles that are optimal for both diagnostics and biomarker discovery. Here, we describe the results and implications from a range of LIPS-antibody profiling studies performed in our laboratory. These include highly sensitive diagnostics for domestic and global pathogens, insights into infection-related diseases, discovery of new biomarkers for human diseases, subcategorization of symptoms and identification of pathogenic autoantibodies against self-proteins. These investigations highlight the types of humoral response profiles associated with different diseases, provide new information related to disease pathogenesis and offer a framework for incorporating LIPS antibody profiling into global health initiatives and disease monitoring.
B 细胞介导的体液免疫反应在许多人类疾病中被触发，包括自身免疫性疾病、癌症、神经和传染病。然而，由于许多免疫分析方法的诊断性能不佳，因此限制了充分利用患者抗体库中的信息进行诊断、监测甚至疾病预测。我们开发了荧光素酶免疫沉淀系统（LIPS），利用发光蛋白来生成高清晰度的抗体谱，这对于诊断和生物标志物发现都是最佳的。在这里，我们描述了我们实验室进行的一系列 LIPS 抗体分析研究的结果和意义。这些研究包括针对国内和全球病原体的高灵敏度诊断、对感染相关疾病的深入了解、发现人类疾病的新生物标志物、对症状进行细分以及鉴定针对自身蛋白的致病性自身抗体。这些研究强调了与不同疾病相关的体液免疫反应谱的类型，提供了与疾病发病机制相关的新信息，并为将 LIPS 抗体分析纳入全球健康计划和疾病监测提供了框架。
